Osteopontin expression in the invasive front stroma of colorectal adenocarcinoma is associated with tumor budding and prognosis
Osteopontin expression in the invasive front stroma of colorectal adenocarcinoma is associated with tumor budding and prognosis
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DOI：
10.1016/j.prp.2022.154190
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发表时间：
2022-11-01
影响因子：
2.8
通讯作者：
Ota, Hiroyoshi
中科院分区：
文献类型：
--
作者：
Nakajima, Tomoyuki;Uehara, Takeshi;Ota, Hiroyoshi
背景：肿瘤出芽（TB）是结直肠癌（CRC）的一个重要预后因素。骨桥蛋白（OPN）在免疫应答、迁移和侵袭、血管生成、上皮-间质转化（EMT）和转移等过程中发挥重要作用。然而，OPN和作为OPN受体的CD 44 v6在TB中的参与尚未阐明。方法：采用免疫组化方法检测83例大肠癌组织中OPN和CD 44 v6的表达，并分析其临床病理特征。骨桥蛋白阳性表达与浸润前沿分化程度及结核分级相关。72例（86.7%）癌细胞CD 44 v6阳性，11例（13.3%）阴性。在OPN阳性组[中位OS：1586（范围，30-2749）天]和OPN阴性组[中位OS：1901（范围，8-2665）天]之间确定了对总生存期（OS）的统计学显著影响（对数秩检验，p = 0.011）。
Background: Tumor budding (TB) is an important prognostic factor in colorectal carcinoma (CRC). Osteopontin (OPN) functions in various processes such as immune response, migration and invasion, angiogenesis, epi-thelial-mesenchymal transition (EMT) and metastasis. However, the involvement of OPN and CD44v6, which is a receptor for OPN, in TB has not been clarified. Therefore, we examined the relationship of OPN with TB in CRC and compared the clinicopathological features.Methods: We investigated the expression of OPN and CD44v6 in 83 cases of CRC by immunostaining and analyzed the clinicopathological features.Results: OPN expression was observed mostly in the cytoplasm of stromal cells such as macrophages and fibro-blasts, and rarely in cancer cells. There was a significant correlation between OPN positivity and the degree of differentiation at the invasive front and TB grade. CD44v6 was positive in cancer cells in 72 cases (86.7 %) and negative in 11 cases (13.3 %). A statistically significant effect on overall survival (OS) was identified between the OPN-positive group [median OS: 1586 (range, 30-2749) days] and the OPN-negative group [median OS: 1901 (range, 8-2665) days] (log-rank test, p = 0.011).Conclusions: OPN analysis in CRC stromal cells may have prognostic implications.