Parsley ubiquitin promoter displays higher activity than the CaMV 35S promoter and the chrysanthemum actin 2 promoter for productive, constitutive, and durable expression of a transgene in Chrysanthemum morifolium
Parsley ubiquitin promoter displays higher activity than the CaMV 35S promoter and the chrysanthemum actin 2 promoter for productive, constitutive, and durable expression of a transgene in Chrysanthemum morifolium
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DOI：
10.1270/jsbbs.19036
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发表时间：
2019-01-01
期刊：
影响因子：
2.4
通讯作者：
Sasaki, Katsutomo
中科院分区：
文献类型：
--
作者：
Kishi-Kaboshi, Mitsuko;Aida, Ryutaro;Sasaki, Katsutomo
菊花（Chrysanthemum morifolium）是世界上最受欢迎的观赏植物之一。遗传转化是改良性状、编辑基因组和研究植物生理学的一种有前途的工具。启动子是高效转化的重要组成部分，决定了转基因表达的水平、位置和时机。花椰菜花叶病毒（CaMV）35S启动子最常用于双子叶植物中，但在烟草或蓝猪耳植物中的效率较低。在此之前，我们首次将欧芹泛素（PcUbi）启动子用于拟南芥，并分析了其在转基因愈伤组织中的活性。为了扩大组成型启动子的种类，我们克隆了肌动蛋白2（CmACT 2）基因的上游区域，并将其启动子活性与几个器官中的35S和PcUbi启动子进行比较，以及其长期培养的耐久性。CmACT 2启动子在愈伤组织中具有比35S启动子更高的活性，但不太持久。PcUbi启动子不仅在愈伤组织中，而且在叶、射线小花和盘状小花中具有最高活性，并且在长期培养后保持其活性。总之，我们提供了有用的信息和一个额外的类型的启动子可用于转基因表达在chromatiums。
The chrysanthemum (Chrysanthemum morifolium) is one of the most popular ornamental plants in the world. Genetic transformation is a promising tool for improving traits, editing genomes, and studying plant physiology. Promoters are vital components for efficient transformation, determining the level, location, and timing of transgene expression. The cauliflower mosaic virus (CaMV) 35S promoter is most frequently used in dicotyledonous plants but is less efficient in chrysanthemums than in tobacco or torenia plants. Previously, we used the parsley ubiquitin (PcUbi) promoter in chrysanthemums for the first time and analyzed its activity in transgenic calli. To expand the variety of constitutive promoters in chrysanthemums, we cloned the upstream region of the actin 2 (CmACT2) gene and compared its promoter activity with the 35S and PcUbi promoters in several organs, as well as its durability for long-term cultivation. The CmACT2 promoter has higher activity than the 35S promoter in calli but is less durable. The PcUbi promoter has the highest activity not only in calli but also in leaves, ray florets, and disk florets, and retains its activity after long-term cultivation. In conclusion, we have provided useful information and an additional type of promoter available for transgene expression in chrysanthemums.