Functional Polymer Brushes on Diamond as a Platform for Immobilization and Electrical Wiring of Biomolecules
Functional Polymer Brushes on Diamond as a Platform for Immobilization and Electrical Wiring of Biomolecules
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DOI：
10.1002/adfm.201202342
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发表时间：
2013-06-20
影响因子：
19
通讯作者：
Garrido, Jose A.
中科院分区：
文献类型：
--
作者：
Reitinger, Andreas A.;Hutter, Naima A.;Garrido, Jose A.
对于电子设备的生物功能化，聚合物刷可以提供一种结合其他常用方法的优点的途径，例如通过自组装单层或涂层聚合物基质固定功能生物分子：高稳定性和负载能力、高效的电子传输和优异的生物相容性。在这里介绍的工作中，聚（甲基丙烯酸）刷是通过金刚石电极上的自引发光接枝和光聚合制备的。在这个简单的过程中，不需要预先接枝引发剂，因为聚合的引发可以通过金刚石表面的氢或氧终止来方便地控制。掺硼纳米晶金刚石作为电极材料提供了极高的化学惰性和稳定性、固有的生物相容性和优异的电化学性能，例如大的可接近电位窗口和低背景电流。作为概念证明，我们演示了通过用氧化还原酶葡萄糖氧化酶和氨甲基二茂铁作为电子介体共价修饰的聚合物刷对葡萄糖进行电流检测。 X 射线光电子能谱和原子力显微镜的表征均表明二茂铁介体的高负载量。电化学循环伏安法一致地显示了二茂铁的多层等效负载以及整个聚合物膜的高效电子转移。总体而言，功能化聚合物刷可以为生物电子和生物传感应用的生物分子固定和电气布线提供一个有前景的平台。
For the biofunctionalization of electronic devices, polymer brushes can provide a route which allows combining the advantages of other commonly used approaches, such as immobilization of functional biomolecules via self assembled monolayers or coated polymer matrices: high stability and loading capacity, efficient electron transport, and excellent biocompatibility. In the work presented here, poly(methacrylic acid) brushes are prepared by self-initiated photografting and photopolymerization on diamond electrodes. In this straightforward process no prior grafting of initiators is required since the initiation of the polymerization can be conveniently controlled by the hydrogen or oxygen termination of the diamond surface. Boron doped nanocrystalline diamond as an electrode material provides extreme chemical inertness and stability, inherent biocompatibility, and superior electrochemical properties, such as the large accessible potential window and low background currents. As a proof of concept we demonstrate the amperometric detection of glucose by polymer brushes covalently modified with the redox enzyme glucose oxidase and aminomethyl ferrocene as electron mediator. Characterization by X-ray photoelectron spectroscopy and atomic force microscopy both indicate a high loading of the ferrocene mediator. Consistently, electrochemical cyclic voltammetry shows a multilayer equivalent loading of ferrocene and highly efficient electron transfer throughout the polymer film. Overall, functionalized polymer brushes can provide a promising platform for the immobilization and electrical wiring of biomolecules for bioelectronic and biosensing applications.