Immortalization in culture of rat cells: a genealogic study.
Immortalization in culture of rat cells: a genealogic study.
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大鼠细胞培养中的永生化：一项谱系研究。
DOI：
10.1093/jnci/74.6.1223
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
R. Konishi
中科院分区：
文献类型：
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作者：
T. Matsumura;M. Hayashi;R. Konishi
用延时电影显微摄影技术研究了体外培养的大鼠肝上皮样细胞永生化的过程。这个过程被证明是多步骤的。首先（在F期），细胞类似于增殖性细胞群中的细胞。大多数细胞在F期终止其增殖生命，而少数细胞获得额外的增殖潜力，随后进入T（过渡）期。在T期，一群细胞保持着增殖潜能，但波动很大：一些细胞进一步获得了额外的增殖潜能，而另一些细胞则失去了增殖潜能。在T期一段时间后，出现了持续分裂的细胞（在I期）。分裂细胞在T期和F期的间期显示细胞呈指数分布，与过渡概率模型一致。然而，I期细胞的分布更窄，通常接近正态分布。根据这些观察，提出了永生化的假设机制。
The process of immortalization of cultured rat liver epithelial-like cells from JAR-2 rats was studied by time-lapse cinemicrography. This process was shown to be multistep. First (in the F-phase), cells were similar to those in a finitely proliferative population. Most cells terminated their proliferative life in the F-phase, while a few gained additional proliferative potential and subsequently entered into the T (transitional)-phase. In the T-phase, a population of cells maintained proliferative potential with a large fluctuation: Some cells further gained additional proliferative potential while others lost it. After a period in the T-phase, continuously dividing cells emerged (in the I-phase). The interphase period of the dividing cells in both the T- and the F-phases showed cells to distribute exponentially, consistent with transitional probability models. However, cells in the I-phase were distributed much more narrowly and were frequently close to a normal distribution. Hypothetical mechanisms of immortalization were presented on the basis of these observations.