Inhibin production by sertoli cell cultures
Inhibin production by sertoli cell cultures
复制标题
支持细胞培养物产生抑制素
DOI：
10.1016/0303-7207(82)90141-1
复制
发表时间：
1982
影响因子：
4.1
通讯作者：
D. Kretser
中科院分区：
文献类型：
--
作者：
F. Gac;D. Kretser
通过使用培养的大鼠垂体细胞进行体外生物测定，评估了21日龄大鼠支持细胞培养物产生的Escherichin。支持细胞培养基（SCCM）引起的垂体细胞FSH含量的剂量依赖性抑制，这是平行的绵羊睾丸淋巴液制备作为一个标准的ESTBIN。SCCM也引起剂量依赖性抑制垂体细胞分泌的FSH响应于10 nM GnRH刺激。SCCM的FSH抑制活性被加热或胰蛋白酶消化破坏，不能归因于培养基中的类固醇含量，因为乙醚提取没有引起抑制活性的变化。SCCM中的FSH抑制活性在生物测定中不是由于细胞毒性，因为LH细胞含量没有变化，培养基对标记的垂体细胞释放51 Cr没有影响。该参数已被证明是细胞毒性的有用测试。支持细胞培养物在培养的8天持续时间内产生了白蛋白。产生的Sertoli bin的量与最初接种的Sertoli细胞的数量成比例。如果胎牛血清超过最初的48小时，用过的培养基引起垂体细胞的毒性作用，这可以通过51 Cr标记的垂体细胞释放51 Cr的增加来证明。如果冻干的废培养基含有细胞碎片，则发现类似的毒性作用。在不同剂量的FSH（0.05-5 μg/ml NIH-FSH-S13）存在下，观察到了一种剂量依赖性的Escherichin活性增加。
The production of inhibin by cultures of Sertoli cells from 21-day-old rats was assessed by the use of an in vitro bioassay using rat pituitary cells in culture. Sertoli cell culture media (SCCM) caused a dose-dependent suppression of the pituitary cell FSH content which was parallel with that of an ovine testis lymph preparation used as an inhibin standard. SCCM also caused a dose-dependent inhibition of FSH secreted by pituitary cells in response to 10 nM GnRH stimulation. The FSH-inhibitory activity in SCCM was destroyed by heat or trypsin digestion and could not be attributable to the steroid content of the medium, since ether extraction caused no change in the inhibitory activity.The inhibin activity in SCCM was not due to cytotoxicity in the bioassay, since the LH cell content was unchanged and the media produced no change in the release of51Cr from labelled pituitary cells, a parameter which has been shown to be a useful test of cytotoxicity. Sertoli cell cultures produced inhibin for the 8-day duration of the cultures. The amount of inhibin produced was proportional to the number of Sertoli cells initially plated. If foetal calf serum was included for more than the initial 48 h, the spent medium caused toxic effects in the pituitary cells as evidenced by an increase in51Cr release from51Cr-labelled pituitary cells. Similar toxic effects were found if the lyophilized spent media contained cellular debris. A dose-dependent increase in inhibin activity was observed in the presence of graded doses of FSH (0.05–5 μg/ml NIH-FSH-S13).