Identification of neurons that express 5-hydroxytryptamine4 receptors in intestine
Identification of neurons that express 5-hydroxytryptamine4 receptors in intestine
复制标题
肠道中表达 5-羟色胺4 受体的神经元的鉴定
作者：
D. Poole;Bo Xu;S. L. Koh;Billie Hunne;I. M. Coupar;H. Irving;K. Shinjo;J. Furness
摘要 5-羟色胺（5-HT）是肠道推进反射的内源性兴奋剂。它部分通过5-HT4受体发挥作用； 5-HT4 受体激动剂是肠道运输的兴奋剂，已在临床使用。药理学和最近的免疫组织化学研究表明，5-HT4 受体存在于肠神经元上，但表达受体的特定神经元尚未确定。在目前的工作中，我们描述了抗 5-HT4 受体抗血清的表征，该血清揭示了肠神经元和胃肠道中其他细胞类型的免疫反应性。使用该抗血清，在大鼠食管粘膜肌层（5-HT4 受体的标准检测组织）中发现了 5-HT4 受体免疫反应性。它也存在于豚鼠和小鼠食道的粘膜肌层中。在豚鼠小肠以及大鼠和小鼠结肠中，5-HT4 受体免疫反应性发生在肠神经元亚群中，包括突出的大神经元。双重染色表明，豚鼠小肠中的这些大神经元也对内在初级传入神经元的两种标记物（细胞质 NeuN 和钙结合蛋白）具有免疫反应性。肌间神经节中的一些肌肉运动神经元对该受体具有免疫反应性，而分泌运动神经元很少表达该受体。免疫反应性也发生在卡哈尔间质细胞中，但在外部肌肉中很弱。蛋白质和 mRNA 的表达已在含有肠神经元的提取物中得到证实。观察结果表明 5-HT4 受体激动剂的一个作用位点是内在的初级传入神经元。
Abstract5-Hydroxytryptamine (5-HT) is an endogenous stimulant of intestinal propulsive reflexes. It exerts its effects partly through 5-HT4 receptors; 5-HT4 receptor agonists that are stimulants of intestinal transit are in clinical use. Both pharmacological and recent immunohistochemical studies indicate that 5-HT4 receptors are present on enteric neurons but the specific neurons that express the receptors have not been determined. In the present work, we describe the characterization of an anti-5-HT4 receptor antiserum that reveals immunoreactivity for enteric neurons and other cell types in the gastrointestinal tract. With this antiserum, 5-HT4 receptor immunoreactivity has been found in the muscularis mucosae of the rat oesophagus, a standard assay tissue for 5-HT4 receptors. It is also present in the muscularis mucosae of the guinea-pig and mouse oesophagus. In guinea-pig small intestine and rat and mouse colon, 5-HT4 receptor immunoreactivity occurs in subpopulations of enteric neurons, including prominent large neurons. Double-staining has shown that these large neurons in the guinea-pig small intestine are also immunoreactive for two markers of intrinsic primary afferent neurons, cytoplasmic NeuN and calbindin. Some muscle motor neurons in the myenteric ganglia are immunoreactive for this receptor, whereas it is rarely expressed by secretomotor neurons. Immunoreactivity also occurs in the interstitial cells of Cajal but is faint in the external muscle. Expression of the protein and mRNA has been confirmed in extracts containing enteric neurons. The observations suggest that one site of action of 5-HT4 receptor agonists is the intrinsic primary afferent neurons.
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DOI：
10.1152/ajpgi.1997.273.2.g422
发表时间：
1997-08
期刊：
The American journal of physiology
影响因子：
--
作者：
Paul P. Bertrand;Wolf A. A. Kunze-Wolf-A.-A.-Kunze-2233345542;J. Bornstein;John B. Furness;M. L. Smith
通讯作者：
Paul P. Bertrand;Wolf A. A. Kunze-Wolf-A.-A.-Kunze-2233345542;J. Bornstein;John B. Furness;M. L. Smith
影响因子：
--
作者：
PAN, H;GALLIGAN, JJ
通讯作者：
GALLIGAN, JJ
豚鼠回肠中 5-羟色胺神经元受体的药理学特征，其性质与 5-羟色胺受体相似。
DOI：
--
发表时间：
1990
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Craig,DA;Clarke,DE
通讯作者：
Clarke,DE
影响因子：
29.4
作者：
Grider, JR;Foxx-Orenstein, AE;Jin, JG
通讯作者：
Jin, JG
DOI：
10.1042/bj3220001
发表时间：
1997-02
期刊：
The Biochemical journal
影响因子：
--
作者：
S. Böhm;E. Grady;N. Bunnett
通讯作者：
S. Böhm;E. Grady;N. Bunnett