Acute and potentially persistent effects of scuba diving on the blood transcriptome of experienced divers
Acute and potentially persistent effects of scuba diving on the blood transcriptome of experienced divers
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DOI：
10.1152/physiolgenomics.00164.2012
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发表时间：
2013-10-01
影响因子：
4.6
通讯作者：
Dujic, Zeljko
中科院分区：
文献类型：
--
作者：
Eftedal, Ingrid;Ljubkovic, Marko;Dujic, Zeljko
在水肺潜水时，潜水员在水下时氧气分压升高，以及上升到水面时由减压引起的气泡对循环系统造成压力。这种由潜水引起的压力可能引发减压疾病，但大多数潜水是无症状的。在这项研究中，我们绘制了潜水员的血液转录组图，目的是识别无症状水肺潜水中受生理应激干扰的基因、生物途径和细胞类型。10名经验丰富的潜水员在连续3天每天在18米深度下潜47分钟，同时呼吸压缩空气之前，放弃了20周的潜水。在第一次和最后一次潜水之前和之后立即收集血液用于微阵列分析，10个匹配的非潜水者提供潜水前固定转录组的对照。对潜水前样本的MetaCore GeneGo分析发现，潜水员中与凋亡、炎症和先天免疫反应相关的基因稳定上调，最显著的是涉及caspase依赖性凋亡的TNFR1途径、通过TLR4信号传导的HSP60/HSP70以及NF-kappa b介导的转录中的基因。潜水导致特异性白细胞的转录模式发生显著变化，CD8 + T淋巴细胞和NK细胞表达的基因下调，中性粒细胞、单核细胞和巨噬细胞表达的基因上调。抗氧化基因上调。在第一次和最后一次潜水后观察到类似的瞬态反应。结果表明，亚致死氧化应激在水肺潜水中引发髓系先天免疫系统，并且广泛的潜水可能导致控制细胞凋亡，炎症和先天免疫反应的途径的持续变化。
During scuba diving, the circulatory system is stressed by an elevated partial pressure of oxygen while the diver is submerged and by decompression-induced gas bubbles on ascent to the surface. This diving-induced stress may trigger decompression illness, but the majority of dives are asymptomatic. In this study we have mapped divers' blood transcriptomes with the aim of identifying genes, biological pathways, and cell types perturbed by the physiological stress in asymptomatic scuba diving. Ten experienced divers abstained from diving for >2 wk before performing a 3-day series of daily dives to 18 m depth for 47 min while breathing compressed air. Blood for microarray analysis was collected before and immediately after the first and last dives, and 10 matched nondivers provided controls for predive stationary transcriptomes. MetaCore GeneGo analysis of the predive samples identified stationary upregulation of genes associated with apoptosis, inflammation, and innate immune responses in the divers, most significantly involving genes in the TNFR1 pathway of caspase-dependent apoptosis, HSP60/HSP70 signaling via TLR4, and NF-kappa B-mediated transcription. Diving caused pronounced shifts in transcription patterns characteristic of specific leukocytes, with downregulation of genes expressed by CD8 + T lymphocytes and NK cells and upregulation of genes expressed by neutrophils, monocytes, and macrophages. Antioxidant genes were upregulated. Similar transient responses were observed after the first and last dive. The results indicate that sublethal oxidative stress elicits the myeloid innate immune system in scuba diving and that extensive diving may cause persistent change in pathways controlling apoptosis, inflammation, and innate immune responses.