Genomic and biological characterization of Newcastle disease viruses isolated from migratory mallards (Anas platyrhynchos)
Genomic and biological characterization of Newcastle disease viruses isolated from migratory mallards (Anas platyrhynchos)
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DOI：
10.1007/s00705-018-3840-8
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发表时间：
2018-08-01
影响因子：
2.7
通讯作者：
Shabbir, Muhammad Zubair
中科院分区：
文献类型：
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作者：
Habib, Momena;Yaqub, Tahir;Shabbir, Muhammad Zubair
鉴于新城疫病毒（ndv）的全球进化动态，必须继续广泛监测、常规监测和鉴定源自天然宿主（水禽）的分离株。在本报告中，我们从临床健康的绿头鸭（Anas platyrhynchos）中分离并鉴定了两株毒力强的NDV毒株。两个分离株的基因组共有15,192个核苷酸，编码6个基因，序列为3‘-NP-P-M-F-HN-L-5’。生物学特性(平均死亡时间：49.5-50小时，EID(50)10（8.5) ml(-1)）和融合蛋白(F)中存在一个典型的裂解位点（112R-R-Q-K-Ra向下箭头F117）证实了这些分离株的速度性。系统发育分析将这两株分离物归类为ii类基因型VII的成员。此外，根据F基因的高变区（375 nt），分离株在亚基因型VIIi内呈聚类。相似指数和平行比较显示与常用疫苗株核苷酸差异较大；LaSota（21%）和Mukteswar（17%）。与不同基因型的代表性菌株（包括疫苗菌株）进行的比较残基分析显示，F和血凝素-神经氨酸酶（HN）蛋白的重要结构域和功能域存在许多替换。总之，这些结果突出了流行ndvv之间的一致进化，支持在水禽中广泛监测该病毒，以更好地阐明流行病学、进化关系及其对商业和后院家禽的影响。
Given the global evolutionary dynamics of Newcastle disease viruses (NDVs), it is imperative to continue extensive surveillance, routine monitoring and characterization of isolates originating from natural reservoirs (waterfowls). In this report, we isolated and characterized two virulent NDV strains from clinically healthy mallard (Anas platyrhynchos). Both isolates had a genome of 15,192 nucleotides encoding six genes in an order of 3'-NP-P-M-F-HN-L-5'. The biological characteristics (mean death time: 49.5-50 hr, EID(50)10(8.5) ml(-1)) and presence of a typical cleavage site in the fusion (F) protein (112R-R-Q-K-Ra down arrow F117) confirmed the velogenic nature of these isolates. Phylogenetic analysis classified both isolates as members of genotype VII within class-II. Furthermore, based upon the hypervariable region of the F gene (375 nt), isolates showed clustering within sub-genotype VIIi. Similarity index and parallel comparison revealed a higher nucleotide divergence from commonly used vaccine strains; LaSota (21%) and Mukteswar (17%). A comparative residues analysis with representative strains of different genotypes, including vaccine strains, revealed a number of substitutions at important structural and functional domains within the F and hemagglutinin-neuraminidase (HN) proteins. Together, the results highlight consistent evolution among circulating NDVs supporting extensive surveillance of the virus in waterfowl to better elucidate epidemiology, evolutionary relationships and their impacts on commercial and backyard poultry.