Superoxide dismutase as a target enzyme for Fe-porphyrin-induced cell death
Superoxide dismutase as a target enzyme for Fe-porphyrin-induced cell death
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DOI：
10.1016/j.jinorgbio.2006.09.029
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发表时间：
2007-02-01
影响因子：
3.9
通讯作者：
Kawakami, Hiroyoshi
中科院分区：
文献类型：
--
作者：
Asayama, Shoichiro;Kasugai, Nobuyoshi;Kawakami, Hiroyoshi
已估计用[5,10-双(N-甲基-4-吡啶基)-15,20-二苯基]卟啉铁(III) (cis-FeM-Py2P2P) 处理的人类癌细胞系的细胞活力。 cis-FeMPy2P2P 是一种体外超氧化物歧化酶 (SOD) 模拟物，在癌细胞系中表现出显着的毒性。这种毒性是由于体内铁卟啉的促氧化特性造成的。我们已经证明，根据用 cis-FeMPy2P2P 处理的癌细胞系的活力计算出的 LD50 值与细胞系的 SOD 活性之间存在关系。此外，反义S-寡核苷酸对SOD的抑制增加了cis-FeMPy2P2P对癌细胞的细胞毒作用。这些结果表明，SOD 是 cis-FeMPy2P2P 作为一类新型抗癌药物诱导细胞死亡的靶酶。 (c) 2006 Elsevier Inc. 保留所有权利。
The cell viability of human cancer cell lines treated with [5,10-bis(N-methyl-4-pyridyl)-15,20-diphenyl]porphinatoiron(III) (cis-FeM-Py2P2P) has been estimated. The cis-FeMPy2P2P is a superoxide dismutase (SOD) mimic in vitro that exhibited a significant toxicity in cancer cell lines. This toxicity is rather due to pro-oxidant properties of the iron-porphyrin in vivo. We have demonstrated that there was the relationship between the LD50 values calculated from the viability of cancer cell lines treated with cis-FeMPy2P2P and the SOD activities of the cell lines. Furthermore, the inhibition of SOD by antisense S-oligonucleotide increased the cytotoxic effect of cis-FeMPy2P2P against cancer cells. These results suggest that SOD is a target enzyme for the cell death induced by cis-FeMPy2P2P as a new class of anticancer agents. (c) 2006 Elsevier Inc. All rights reserved.