Flow Dynamics and HSPC Homing in Bone Marrow Microvessels.
Flow Dynamics and HSPC Homing in Bone Marrow Microvessels.
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DOI：
10.1016/j.celrep.2017.01.042
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发表时间：
2017-02-14
期刊：
影响因子：
8.8
通讯作者：
Adams RH
中科院分区：
文献类型：
--
作者：
Bixel MG;Kusumbe AP;Ramasamy SK;Sivaraj KK;Butz S;Vestweber D;Adams RH
在单个动脉血管和窦状毛细血管水平上测量流速对于理解骨髓血管系统中造血干细胞和祖细胞归巢的动力学是至关重要的。我们已经开发了两种互补的活体双光子成像方法，通过捕获红细胞的运动来确定多个血管段中的血流动力学和速度。通过颅窗进行高分辨率时空测量，以确定流动血细胞的短时动态和重复中心线扫描，以获得详细的血流剖面图和血流动力学参数。此外，我们观察了单个造血干细胞和祖细胞的归巢，并获得了有关其归巢行为的详细信息。通过我们的成像设置，我们确定了小动脉血管和高度分支的窦状毛细血管中细胞分辨率下的血流模式、血流速度和壁面切应力，以及造血干细胞和祖细胞归巢的细胞动力学。颅骨骨髓微血管系统的详细3D重建骨微血管中细胞分辨率下的血流动力学动脉和高度分支的窦状隙中血流动力学参数的详细血流图HSPC归巢至骨髓窦状隙的细胞动力学Bixel et al.使用活体双光子成像以细胞分辨率和血液动力学参数确定骨髓微血管系统中单个动脉血管和窦状毛细血管的血流模式。他们报告了造血干细胞和祖细胞归巢到高度分支的骨髓血窦的动力学的详细信息。
Measurements of flow velocities at the level of individual arterial vessels and sinusoidal capillaries are crucial for understanding the dynamics of hematopoietic stem and progenitor cell homing in the bone marrow vasculature. We have developed two complementary intravital two-photon imaging approaches to determine blood flow dynamics and velocities in multiple vessel segments by capturing the motion of red blood cells. High-resolution spatiotemporal measurements through a cranial window to determine short-time dynamics of flowing blood cells and repetitive centerline scans were used to obtain a detailed flow-profile map with hemodynamic parameters. In addition, we observed the homing of individual hematopoietic stem and progenitor cells and obtained detailed information on their homing behavior. With our imaging setup, we determined flow patterns at cellular resolution, blood flow velocities and wall shear stress in small arterial vessels and highly branched sinusoidal capillaries, and the cellular dynamics of hematopoietic stem and progenitor cell homing. Detailed 3D reconstruction of the calvarial bone marrow microvasculature Blood flow dynamics at cellular resolution in bone microvessels Detailed flow map with hemodynamic parameters in arteries and highly branched sinusoids Cellular dynamics of HSPC homing to bone marrow sinusoids Bixel et al. use intravital two-photon imaging to determine blood flow patterns at cellular resolution and hemodynamic parameters in individual arterial vessels and sinusoidal capillaries in the bone marrow microvasculature. They report detailed information on the dynamics of hematopoietic stem and progenitor cell homing to highly branched bone marrow sinusoids.