MicroRNA-93 inhibits ischemia-reperfusion induced cardiomyocyte apoptosis by targeting PTEN.
MicroRNA-93 inhibits ischemia-reperfusion induced cardiomyocyte apoptosis by targeting PTEN.
复制标题
DOI：
10.18632/oncotarget.8941
复制
发表时间：
2016-05-17
期刊：
影响因子：
--
通讯作者：
Gao AM
中科院分区：
文献类型：
--
作者：
Ke ZP;Xu P;Shi Y;Gao AM
MicroRNAs参与了包括心肌缺血/再灌注（I/R）损伤在内的一些生物学和病理过程。最近的研究结果表明，miR-93可能对缺血性心脏病提供潜在的心脏保护作用。本研究旨在探讨miR-93在I/ r诱导的心肌细胞损伤中的作用及其可能的机制。在这项研究中，我们发现缺氧/再氧化（H/R）显著增加LDH释放、MDA含量、ROS生成和内质网应激（ERS）介导的心肌细胞凋亡，而与miR-93 mimic共转染可减弱这些凋亡。磷酸酶和紧张素同源物（PTEN）被确定为miR-93的靶基因。此外，miR-93 mimic在H/R下显著增加p-Akt水平，LY294002部分释放p-Akt。此外，Ad-miR-93在体内还能减轻心肌I/R损伤，表现为LDH和CK水平降低，梗死面积减少，细胞凋亡减少。综上所述，我们的研究结果表明miR-93可以通过抑制PI3K/AKT/PTEN信号传导来保护I/ r诱导的心肌细胞凋亡。
MicroRNAs have been implicated in some biological and pathological processes, including the myocardial ischemia/reperfusion (I/R) injury. Recent findings demonstrated that miR-93 might provide a potential cardioprotective effect on ischemic heart disease. This study was to investigate the role of miR-93 in I/R-induced cardiomyocyte injury and the potential mechanism. In this study, we found that hypoxia/reoxygenation (H/R) dramatically increased LDH release, MDA contents, ROS generation, and endoplasmic reticulum stress (ERS)-mediated cardiomyocyte apoptosis, which were attenuated by co-transfection with miR-93 mimic. Phosphatase and tensin homolog (PTEN) was identified as the target gene of miR-93. Furthermore, miR-93 mimic significantly increased p-Akt levels under H/R, which was partially released by LY294002. In addtion, Ad-miR-93 also attenuated myocardial I/R injury in vivo, manifested by reduced LDH and CK levels, infarct area and cell apoptosis. Taken together, our findings indicates that miR-93 could protect against I/R-induced cardiomyocyte apoptosis by inhibiting PI3K/AKT/PTEN signaling.
登录
查看更多内容
木薯 ZN1 对木质纤维素预处理所得糠醛和 5-羟甲基糠醛生物降解的转录分析
影响因子：
6.3
作者：
Wang X;Gao Q;Bao J
通讯作者：
Bao J
影响因子：
4.7
作者：
Singh, Bhupendra;Ronghe, Amruta M.;Bhat, Hari K.
通讯作者：
Bhat, Hari K.
DOI：
10.3724/sp.j.1263.2012.00028
发表时间：
2012-03
期刊：
Journal of geriatric cardiology : JGC
影响因子：
--
作者：
Liu LF;Liang Z;Lv ZR;Liu XH;Bai J;Chen J;Chen C;Wang Y
通讯作者：
Wang Y
Elatoside C 通过减少内质网应激来防止 H9c2 心肌细胞缺氧/复氧诱导的细胞凋亡，部分取决于 STAT3 激活
影响因子：
7.2
作者：
Wang, Min;Meng, Xiang-bao;Sun, Xiao-bo
通讯作者：
Sun, Xiao-bo
影响因子：
7.4
作者：
Zheng L;Zhang Y;Liu Y;Zhou M;Lu Y;Yuan L;Zhang C;Hong M;Wang S;Li X
通讯作者：
Li X