A cluster of six tRNA genes in Drosophila mitochondrial DNA that includes a gene for an unusual tRNAserAGY.
A cluster of six tRNA genes in Drosophila mitochondrial DNA that includes a gene for an unusual tRNAserAGY.
复制标题
果蝇线粒体 DNA 中的六个 tRNA 基因簇，其中包括一个不寻常的 tRNAserAGY 基因。
DOI：
10.1093/nar/12.5.2367
复制
发表时间：
1984
影响因子：
14.9
通讯作者：
D. Wolstenholme
中科院分区：
文献类型：
--
作者：
D. Clary;D. Wolstenholme
URF3、tRNAala、tRNAarg、tRNAason、tRNAserAGY、tRNAglu、tRNAphe和URF5基因的羧基末端片段已经在果蝇mtDNA分子的一个序列片段中被鉴定。这些基因按照给定的顺序出现。URF5和tRNAphe基因的转录方向与复制方向相同，而URF3和其余5个tRNA基因的转录方向相反。这些基因在雅库巴血吸虫和哺乳动物mtDNA分子中的相对排列有相当大的差异。在tRNAserAGY基因中，取代了二氢尿苷臂的是一个11个核苷酸环，其内似乎不太可能形成二级结构，并且可变环(6个核苷酸)和T Phi C环(9个核苷酸)都比其他任何雅库巴tRNA基因的大。由于现有的证据与果蝇线粒体遗传密码中指定丝氨酸而不是精氨酸的AGA密码子一致，因此有可能雅库巴tRNAserAGY基因的5‘GCU反密码子既能识别AGA密码子，又能识别AGY密码子。
Genes for URF3, tRNAala, tRNAarg, tRNAasn, tRNAserAGY, tRNAglu, tRNAphe, and the carboxyl terminal segment of the URF5 gene have been identified within a sequenced segment of the mtDNA molecule of Drosophila yakuba. The genes occur in the order given. The URF5 and tRNAphe genes are transcribed in the same direction as replication while the URF3 and remaining five tRNA genes are transcribed in the opposite direction. Considerable differences exist in the relative arrangement of these genes in D. yakuba and mammalian mtDNA molecules. In the tRNAserAGY gene an eleven nucleotide loop, within which secondary structure formation seems unlikely, replaces the dihydrouridine arm, and both the variable loop (six nucleotides) and the T phi C loop (nine nucleotides) are larger than in any other D. yakuba tRNA gene. As available evidence is consistent with AGA codons specifying serine rather than arginine in the Drosophila mitochondrial genetic code, the possibility is considered that the 5'GCU anticodon of the D. yakuba tRNAserAGY gene can recognize AGA as well as AGY codons.