Amplicon sequencing of variable 16S rRNA and ITS2 regions reveal honeybee susceptibility to diseases resulted of their dietary preferences under anthropogenic landforms
Amplicon sequencing of variable 16S rRNA and ITS2 regions reveal honeybee susceptibility to diseases resulted of their dietary preferences under anthropogenic landforms
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DOI：
10.1101/2021.01.28.428626
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发表时间：
2021-01
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通讯作者：
A. Ptaszyńska;Przemysław Latoch;P. J. Hurd;A. Polaszek;Joanna Michalska-Madej;Łukasz Grochowalski;Dominik Strapagiel;S. Gnat;Daniel Załuski;M. Gancarz;R. Rusinek;Patcharin Krutmuang;R. Martín Hernández;M. Pascual;A. Starosta
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作者：
A. Ptaszyńska;Przemysław Latoch;P. J. Hurd;A. Polaszek;Joanna Michalska-Madej;Łukasz Grochowalski;Dominik Strapagiel;S. Gnat;Daniel Załuski;M. Gancarz;R. Rusinek;Patcharin Krutmuang;R. Martín Hernández;M. Pascual;A. Starosta
欧洲蜜蜂和亚洲蜜蜂是重要的农作物传粉者。微生物组研究可以提供与环境变化和植物可获得性有关的这些昆虫的健康和适合性的复杂信息。16S rRNA可变区和内部转录间隔区(ITSS)的扩增序列可以识别代谢组。这些方法为监测从蜜蜂肠道中分离出来的未培养微生物提供了一种工具。它们还有助于监测肠道真菌的组成，有趣的是，还可以监测昆虫采集的花粉。在这里，我们提供了从波兰、英国、西班牙、希腊和泰国的城市地区的人为地貌中收集的不同时间点的蜜蜂的16S rRNA和ITS2区域的扩增序列数据。我们分析了蜜蜂肠道以及真菌和花粉的微生物组成。结果表明，样品间的差异主要受细菌、植物花粉和真菌的影响。此外，以主要以糖为基础的蜜露饮食喂养的蜜蜂更容易感染真菌病原体(脑微孢子虫)和新食菌素。最后，在整个养蜂季节，蜜蜂转换到冬季世代(寿命较长的觅食蜜蜂)的时期对饮食扰动最敏感，因此病原体的攻击最敏感。在现代拟人化的环境中，蜜蜂的进化适应可能无法让它们受益。
European Apis mellifera and Asian Apis cerana honeybees, are essential crop pollinators. Microbiome studies can provide complex information on health and fitness of these insects in relation to environmental changes, and plant availability. Amplicon sequencing of variable regions of 16S rRNA and internally transcribed spacers (ITSs) allow identification of the metabiome. These methods provide a tool for monitoring otherwise uncultured microbes isolated from the gut of the honeybees. They also help monitor the composition of the gut fungi and, intriguingly, pollens collected by the insect. Here, we present data from amplicon sequencing of the 16S rRNA and ITS2 regions from honeybees collected at various time points from anthropogenic landforms as urban areas in Poland, UK, Spain, Greece, and Thailand. We have analysed microbial composition of honeybee intestine as well as fungi and pollens. We conclude that differences between samples were mainly influenced by the bacteria, plant pollens and fungi, respectively. Moreover, honeybees feeding on a honeydew diet, mainly based on sugars, were more prone to fungal pathogens (Nosema ceranae) and neogregarines. Finally, the period when honeybees switch to the winter generation (longer-lived forager honeybees) is the most sensitive to diet perturbations and hence pathogens attack, for the whole beekeeping season. It is possible that evolutionary adaptation of bees fails to benefit them in the modern anthropomorphised environment.