Histological spectrum of mycophenolate mofetil-related colitis: association with apoptosis
Histological spectrum of mycophenolate mofetil-related colitis: association with apoptosis
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DOI：
10.1111/his.12222
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发表时间：
2013-11-01
期刊：
影响因子：
6.4
通讯作者：
Delladetsima, Ioanna
中科院分区：
文献类型：
--
作者：
Liapis, George;Boletis, John;Delladetsima, Ioanna
目的本研究的主要目的是确定诊断组织学特征霉酚酸酯（MMF）相关性结肠炎与隐窝上皮细胞turning.Methods和resultsThe检查材料包括43例结肠活检肾移植受者与霉酚酸酯管理和持续性腹泻。33例患者出现了与MMF相关的结肠炎，10例无明显变化。组织学检查结果进行评分，并与凋亡指数（AI）和增殖率（PR）的隐窝上皮细胞TUNEL法和Ki-67免疫表达检测。10例克罗恩病和10例溃疡性结肠炎作为对照组。隐窝变形和丢失以及细胞凋亡增加构成了主要特征，其程度和组合导致炎症性肠病（IBD）样（82%）或移植物抗宿主病样模式（18%）。高AI与中度和重度隐窝变形相关，而AI值与对照组相比显著升高（P
AimsThe main purpose of this study was to define diagnostic histological characteristics of mycophenolate mofetil (MMF)-related colitis in association with crypt epithelial cell turnover.Methods and resultsThe examined material included 43 colonic biopsies from renal transplant recipients with MMF administration and persistent diarrhoea. Thirty-three cases showed MMF-related colitis, while 10 showed no significant changes. The histological findings were scored and correlated with the apoptotic index (AI) and with the proliferation rate (PR) of the crypt epithelium examined by TUNEL assay and Ki-67 immunoexpression. Ten cases of Crohn disease and 10 of ulcerative colitis were used as comparative groups. Crypt distortion and loss as well as increased apoptosis constituted the main features, their degree and combination leading either to an inflammatory bowel disease (IBD)-like (82%) or to a graft-versus-host disease-like pattern (18%). A high AI was associated more frequently with moderate and severe crypt distortion, while the values were significantly higher compared with the control groups (P