Simultaneous liquid-chromatographic determination of three antiarrhythmic drugs: disopyramide, lidocaine, and quinidine.
Simultaneous liquid-chromatographic determination of three antiarrhythmic drugs: disopyramide, lidocaine, and quinidine.
复制标题
同时液相色谱法测定三种抗心律失常药物：丙吡胺、利多卡因和奎尼丁。
DOI：
10.1093/clinchem/26.2.0197
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发表时间：
1980
影响因子：
9.3
通讯作者：
R. Mccomb
中科院分区：
文献类型：
--
作者：
J. Flood;G. Bowers;R. Mccomb
We report a common methodology for determining three antiarrhythmic drugs: disopyramide, lidocaine, and quinidine. Alkalinized serum and internal standard (p-chlorodisopyramide) are extracted into dichloromethane, the organic phase is evaporated, and the redissolved residue is injected onto a reversed-phase column (micron Bondapack C18). Quantitation is via peak-height ratios of analyte vs internal standard (as detected at 205 nm) referenced to a serum-based multiple-drug standard. A mobile phase of 30 mmol/L phosphate buffer and acetonitrile (72/28 by vol) is used. These conditions yiel; optimum separation and band symmetry for the analytes and some of their metabolites. Crucial factors in this simultaneous assay include pH of the mobile phase and injected solution, extraction time, and evaporation technique. Day-to-day precision (CV) for all drugs was less than 5%, and correlation with other assay techniques for each drug is reported. The method enables more efficient use of personnel and instrumentation without sacrificing analytical quality.
