Ectopic expression of BrIQD35 promotes drought stress tolerance in Nicotiana benthamiana
Ectopic expression of BrIQD35 promotes drought stress tolerance in Nicotiana benthamiana
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BrIQD35 的异位表达促进本塞姆氏烟草的干旱胁迫耐受性
DOI：
10.1111/plb.13425
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发表时间：
2022
期刊：
影响因子：
3.9
通讯作者：
Li Ying
中科院分区：
文献类型：
--
作者：
Yuan Jingping;Yu Zhanghong;Li Yan;Shah Hamad;Xiao dong;Hou Xilin;Li Ying
植物tiqdgene家族对多种胁迫有反应。在本研究中，我们研究了中国大白菜中briqd35基因的结构特征和功能。briqd35被克隆并显示包含一个IQ基序。briqd35在大白菜中的瞬时表达表明，briqd35定位于质膜上，在干旱和盐胁迫下表达量显著上调。为了进一步确定briqd35的功能，我们在烟叶中异种过表达briqd35。盐胁迫下，briqd35过表达量与野生型（WT）植物间无显著差异。在干旱胁迫下，benthamiana比briqd35‐过表达的植物更容易萎蔫。由于IQ基序已被注释为CaM结合位点，因此使用酵母双杂交试验来探索BrIQD35与CaM之间的相互作用。结果表明，BrIQD35与CaMb相互作用弱，但与CaMa不相互作用，表明BrIQD35可能通过Ca2+‐CaMb途径起作用。这一发现揭示了一个与耐旱有关的新基因，对大白菜的植物育种和品质改良具有重要意义。
The plantIQDgene family is responsive to a variety of stresses. In this study, we studied the structural features and functions of the geneBrIQD35in Chinese cabbage, a member of theIQDgene family.BrIQD35was cloned and shown to contain an IQ motif. Transient expression ofBrIQD35indicated that it was localized on the plasma membrane and was significantly upregulated under drought and salt stress in Chinese cabbage. To further identify the function ofBrIQD35, it was heterologously overexpressed inNicotiana benthamiana. Although there was no significant difference betweenBrIQD35‐overexpressed and wild‐type (WT) plants under salt stress, WTN.benthamianashowed more wilting than theBrIQD35‐overexpressed plants under drought stress.Since the IQ motif has been annotated as a CaM binding site, yeast two‐hybrid assays were used to explore the interaction between BrIQD35 and CaM. The results indicated that BrIQD35 interacts weakly with CaMb, but not with CaMa, suggesting that BrIQD35 may function through the Ca2+‐CaMb pathway.The findings reveal a novel gene involved in drought tolerance, which is important for plant breeding and quality improvement for Chinese cabbage.