GROWTH OF DIPLOID-CELLS FROM BREAST CANCERS
GROWTH OF DIPLOID-CELLS FROM BREAST CANCERS
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DOI：
10.1016/0165-4608(85)90077-9
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发表时间：
1985-01-01
影响因子：
--
通讯作者：
HACKETT, AJ
中科院分区：
文献类型：
--
作者：
WOLMAN, SR;SMITH, HS;HACKETT, AJ
细胞培养取自[人]正常和癌变的乳腺组织，以及转移性乳腺组织，方法是选择相对黏附的上皮聚集体。第一代或第二代培养的核型分析显示，主要是正常的二倍体细胞。非克隆像差在肿瘤来源的培养中比在正常培养中更常见。三种起源于转移的培养物具有异常克隆的特征。基于DNA含量的观察表明，相当一部分乳腺癌主要由二倍体细胞组成。它们与肿瘤积液的长期培养中的染色体发现有很大的不同，因此，强调了在单个起源组织--乳腺的肿瘤中可以发现的核型多样性。
Cell cultures were derived from [human] normal and cancerous breast tissues and from metastases by methods that selected for relatively adherent eithelial aggregates. Karyotypic analyses of 1st or 2nd passage cultures yielded predominantly normal diploid cells. Nonclonal aberrations were more common in tumor-derived than in normal cultures. Three of the cultures that originated from metastases were characterized by abnormal clones. Observations based on DNA content, indicate that a considerable fraction of breast cancers are composed predominantly of diploid cells. They differ greatly from chromosomal findings in long-term cultures of tumor effusions and, thus, emphasize the karyotypic diversity that can be found in tumors from a single tissue of origin, the breast.