Carbohydrate metabolism of the isolated perfused liver of normal and genetically obese--hyperglycaemic (ob-ob) mice.
Carbohydrate metabolism of the isolated perfused liver of normal and genetically obese--hyperglycaemic (ob-ob) mice.
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正常和遗传性肥胖高血糖（ob-ob）小鼠离体灌注肝脏的碳水化合物代谢。
DOI：
10.1042/bj1250773
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发表时间：
1971
期刊：
影响因子：
--
通讯作者：
A. Beloff
中科院分区：
文献类型：
--
作者：
J. Elliott;D. Hems;A. Beloff
1.发展了一种小鼠肝脏灌流技术，并研究了正常小鼠和遗传性肥胖小鼠肝脏中碳水化合物代谢的一些方面，这些小鼠都是隐性基因ob纯合子。2.小鼠肝脏灌流后的糖异生速度明显快于已报道的小鼠肝脏切片，尤其是乳酸和丙酮酸。3.饲喂肥胖小鼠肝脏糖原分解为葡萄糖的速率较快，但分解为乳酸的速率不大。4.饥饿20h的肥胖小鼠肝脏糖原合成能力增强。5.与瘦小鼠相比，喂食或饥饿肥胖小鼠肝脏中多种底物的糖异生能力没有明显改变。6.这些结果表明，肥胖小鼠的高血糖是肝脏通过糖原分解增加而引起的，而肥胖小鼠的肝糖原是通过饮食中葡萄糖的合成来维持的。
1. A technique for perfusion of the mouse liver has been developed, and aspects of carbohydrate metabolism have been investigated in the perfused liver of normal and genetically obese mice, homozygous for the recessive gene ob. 2. Rates of gluconeogenesis in perfused mouse liver were faster than those reported for slices of mouse liver, particularly from lactate and pyruvate. 3. The rate of glycogen breakdown to glucose, but not to lactate, was faster in liver from fed obese mice. 4. The capacity for glycogen synthesis from glucose was enhanced in liver from 20h-starved obese mice. 5. The capacity for gluconeogenesis from a number of substrates was not significantly altered in livers from fed or starved obese mice when compared with that of lean mice. 6. These results suggest that the liver contributes to the hyperglycaemia of the obese mice by increased glycogenolysis, and that liver glycogen in obese mice is maintained by synthesis from dietary glucose.