Convergent gene loss following gene and genome duplications creates single-copy families in flowering plants
Convergent gene loss following gene and genome duplications creates single-copy families in flowering plants
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DOI：
10.1073/pnas.1300127110
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发表时间：
2013-02-19
影响因子：
11.1
通讯作者：
Van de Peer, Yves
中科院分区：
文献类型：
--
作者：
De Smet, Riet;Adams, Keith L.;Van de Peer, Yves
通过复制获得基因的重要性早已被认识到。相比之下，基因丢失的重要性直到最近才引起人们的注意。事实上，对从植物到蠕虫和人类的生物体的研究表明，某些基因的复制可能比其他基因的复制更容易被容忍。在这里，我们进行了一项大规模的研究，以调查是否存在的复制抗性基因的测序基因组中的20种开花植物。我们证明，有一个大的基因集，收敛恢复到单拷贝状态后，多个全基因组和较小规模的重复事件。我们排除了这样的模式可以解释为随机基因丢失的可能性，因此提出，有选择的压力，以保持这样的基因作为单例。被子植物单拷贝基因不包含基因组的随机部分，而是通常参与在所有真核生物中高度保守的基本管家功能，这进一步证实了这一点。此外，单拷贝基因通常比非单拷贝基因在更多组织中表达更高，并且它们表现出更高的序列保守性。最后，我们提出了不同的假设来解释它们对重复的抵抗。
The importance of gene gain through duplication has long been appreciated. In contrast, the importance of gene loss has only recently attracted attention. Indeed, studies in organisms ranging from plants to worms and humans suggest that duplication of some genes might be better tolerated than that of others. Here we have undertaken a large-scale study to investigate the existence of duplication-resistant genes in the sequenced genomes of 20 flowering plants. We demonstrate that there is a large set of genes that is convergently restored to single-copy status following multiple genome-wide and smaller scale duplication events. We rule out the possibility that such a pattern could be explained by random gene loss only and therefore propose that there is selection pressure to preserve such genes as singletons. This is further substantiated by the observation that angiosperm single-copy genes do not comprise a random fraction of the genome, but instead are often involved in essential housekeeping functions that are highly conserved across all eukaryotes. Furthermore, single-copy genes are generally expressed more highly and in more tissues than non-single-copy genes, and they exhibit higher sequence conservation. Finally, we propose different hypotheses to explain their resistance against duplication.