Levels of Blood Organophosphorus Flame Retardants and Association with Changes in Human Sphingolipid Homeostasis
Levels of Blood Organophosphorus Flame Retardants and Association with Changes in Human Sphingolipid Homeostasis
复制标题
血液有机磷阻燃剂的水平及其与人体鞘脂稳态变化的关系
DOI：
10.1021/acs.est.6b02474
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发表时间：
2016
影响因子：
11.4
通讯作者：
Hu Jianying
中科院分区：
文献类型：
--
作者：
Zhao Fanrong;Wan Yi;Zhao Haoqi;Hu Wenxin;Mu Di;Webster Thomas F.;Hu Jianying
虽然最近的毒理学研究表明，有机磷阻燃剂（OPFR）可能会破坏鞘脂的体内平衡，但目前缺乏流行病学证据。在这项研究中，共招募了257名来自中国深圳的参与者。首次采用超高效液相色谱-串联质谱法同时测定了人体血液样品中11种OPFR。在至少90%的参与者中可检测到六种OPFR，磷酸三丁酯（TNBP）、磷酸2-乙基己基二苯基酯（EHDPP）、磷酸三（2-氯异丙基）酯（TCIPP）、磷酸三（2-丁氧基乙基）酯（TBOEP）、磷酸三乙酯（TEP）和TPHP，中位浓度分别为37.8、1.22、0.71、0.54、0.49和0.43 ng/mL。在EHDPP、TPHP、TNBP、TBOEP、TEP和TCIPP的最高四分位数中，鞘磷脂（SM）水平为45.3% [95%置信区间; 38.1%、53.0%]、51.9%（45.5%、58.6%）、153.6%（145.1%、162.3%）、20.6%校正协变量后，分别比最低四分位数高14.5%，27.0%，59.0%，52.1%，66.2%，62.8%，55.2%，70.6%。EHDPP、TPHP和TNBP最高四分位数的鞘氨醇1-磷酸（S1 P）水平分别比最低四分位数低36%（− 39%，−33%）、16%（− 19%，−14%）和36%（− 38%，−33%）。当连续模拟暴露时，出现了类似的模式。我们首次发现OPFR与人类鞘脂稳态变化之间的关联。
While a recent toxicological study has shown that organophosphorus flame retardants (OPFRs) may disrupt sphingolipid homeostasis, epidemiologic evidence is currently lacking. In this study, a total of 257 participants were recruited from Shenzhen, China. Eleven OPFRs were for the first time simultaneously determined in the human blood samples by ultraperformance liquid chromatography and tandem mass spectrometry. Six OPFRs, tributyl phosphate (TNBP), 2-ethylhexyl diphenyl phosphate (EHDPP), tris(2-chloroisopropyl) phosphate (TCIPP), tris(2-butoxyethyl) phosphate (TBOEP), triethyl phosphate (TEP), and TPHP, were detectable in at least 90% of participants, with median concentrations of 37.8, 1.22, 0.71, 0.54, 0.49, and 0.43 ng/mL, respectively. Sphingomyelin (SM) levels in the highest quartile of EHDPP, TPHP, TNBP, TBOEP, TEP, and TCIPP were 45.3% [95% confidence interval; 38.1%, 53.0%], 51.9% (45.5%, 58.6%), 153.6% (145.1%, 162.3%), 20.6% (14.5%, 27.0%), 59.0% (52.1%, 66.2%), and 62.8% (55.2%, 70.6%) higher than those in the lowest quartile, respectively, after adjusting for covariates. Sphingosine 1-phosphate (S1P) levels in the highest quartile of EHDPP, TPHP, and TNBP were 36% (−39%, −33%), 16% (−19%, −14%), and 36% (−38%, −33%) lower than those in the lowest quartile, respectively. A similar pattern emerged when exposures were modeled continuously. We for the first time found the associations between OPFRs and changes in human sphingolipid homeostasis.