Rapid HPLC/DAD/MS method to determine phenolic acids, glycoalkaloids and anthocyanins in pigmented potatoes (Solanum tuberosum L) and correlations with variety and geographical origin
Rapid HPLC/DAD/MS method to determine phenolic acids, glycoalkaloids and anthocyanins in pigmented potatoes (Solanum tuberosum L) and correlations with variety and geographical origin
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DOI：
10.1016/j.foodchem.2010.09.009
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发表时间：
2011-03-15
期刊：
影响因子：
8.8
通讯作者：
Mulinacci, Nadia
中科院分区：
文献类型：
--
作者：
Ieri, Francesca;Innocenti, Marzia;Mulinacci, Nadia
本研究采用单一方法同时测定13个色素果皮品种的酚酸、花青素和糖生物碱。提出了三种化合物的单一穷举提取方法和单一HPLC分析方法。从定量的角度来看，这三类代谢物的日内和日间RSD值均< 5.7%。关于不同种植区域的影响，在意大利南部海拔1000米的地理区域发现块茎中花青素浓度最高，果肉中乙醇碱含量较低。主成分分析（PCA）不能区分酚酸类的不同聚类，而花青素类的散点图将品种聚为5个不同的类群。这些色素可以作为该品种的“指纹”。
In the present study a single procedure to simultaneously determine phenolic acids, anthocyanins and glycoalkaloids was applied in 13 cultivars all with pigmented peel. A single exhaustive extraction for the three classes of compounds and a single analytical HPLC method were proposed. From a quantitative point of view, the RSD values from intra and inter-day assays were < 5.7% for the three classes of metabolites. Concerning the influence of different growing areas, the highest anthocyanin concentrations and the lower glycolkaloid content in the pulp were found in tubers from the geographical area at 1000 m a.s.l in southern Italy. Principal Component Analysis (PCA) did not allow the discrimination of different clusters when applied to phenolic acids, while the scatterplot obtained with regard to the anthocyanin profiles clustered the cultivars into five distinct groups. These pigments can be proposed as "fingerprints" of the cultivar.