Formation of a barrier by brain microvessel endothelial cells in culture.
Formation of a barrier by brain microvessel endothelial cells in culture.
复制标题
培养物中脑微血管内皮细胞形成屏障。
DOI：
--
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
G. Goldstein
中科院分区：
文献类型：
--
作者：
K. Dorovini‐Zis;P. Bowman;A. Betz;G. Goldstein
从牛脑微血管中分离出的内皮细胞 (EC) 在原代培养物中生长时会产生连续的单层细胞。 EC 通过紧密连接连接在一起，并且含有很少的胞饮小泡。辣根过氧化物酶 (HRP) 无法穿透该体外屏障系统。将细胞暴露于 1.6 M 阿拉伯糖中，随着 HRP 渗透单层，产生紧密连接的可逆分离，其模式类似于将高渗溶液注入颈动脉后在动物中观察到的模式。培养中脑微血管细胞的行为表明它们保留了对血脑屏障形成重要的特性。
Endothelial cells (EC) isolated from bovine brain microvessels produce a continuous monolayer when grown in primary culture. The EC are joined together by tight junctions and contain few pinocytotic vesicles. Horseradish peroxidase (HRP) is unable to penetrate this in vitro barrier system. Exposure of the cells to 1.6 M arabinose produces a reversible separation of the tight junctions with penetration of HRP across the monolayer in a pattern similar to that observed in animals after infusion of hyperosmotic solutions into the carotid artery. The behavior of brain microvascular cells in culture suggest that they retain properties important to the formation of the blood-brain barrier.