Oxidative demethylation of DNA and RNA mediated by non-heme iron-dependent dioxygenases.
Oxidative demethylation of DNA and RNA mediated by non-heme iron-dependent dioxygenases.
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DOI：
10.1002/asia.201402148
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发表时间：
2014-08
期刊：
影响因子：
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通讯作者：
Lining Lu;Chenxu Zhu;B. Xia;C. Yi
中科院分区：
文献类型：
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作者：
Lining Lu;Chenxu Zhu;B. Xia;C. Yi
DNA/RNA甲基化可以由甲基转移酶产生，因此在调节细胞过程中起关键作用;或者，核酸甲基化可以由甲基化剂产生，如果不修复，则具有细胞毒性/致突变性。由非血红素铁依赖性双加氧酶介导的氧化去甲基化是逆转调节甲基化的细胞作用或甲基化损伤的细胞毒性/致突变作用的有效方法。在这篇焦点综述中，我们总结了以泰特和AlkB家族蛋白为例的核酸双加氧酶研究的最新进展，重点是最近文献中的化学见解。这些双加氧酶的化学机制的比较表明，在机制的差异也有助于显着不同的生物功能。
DNA/RNA methylation can be generated by methyltransferases and thus plays a critical role in regulating cellular processes; alternatively, nucleic acid methylation can be produced by methylation agents and is cytotoxic/mutagenic if left unrepaired. Oxidative demethylation mediated by non-heme iron-dependent dioxygenases is an efficient way to reverse either the cellular roles of regulatory methylation or the cytotoxic/mutagenic effects of methylation damage. In this Focus Review we summarize recent advances in the study of nucleic acid dioxygenases exemplified by the TET and AlkB family proteins, with an emphasis on chemical insights from the recent literature. Comparison of the chemical mechanisms of these dioxygenases revealed that differences in the mechanism also contribute significantly to their distinct biological functions.