SEROTYPING HIV TYPE-1 BY ANTIBODY-BINDING TO THE V3 LOOP - RELATIONSHIP TO VIRAL GENOTYPE
SEROTYPING HIV TYPE-1 BY ANTIBODY-BINDING TO THE V3 LOOP - RELATIONSHIP TO VIRAL GENOTYPE
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DOI：
10.1089/aid.1994.10.1379
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发表时间：
1994-11-01
影响因子：
1.5
通讯作者：
KORBER, B
中科院分区：
文献类型：
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作者：
CHEINGSONGPOPOV, R;LISTER, S;KORBER, B
我们已经研究了代表HIV-1主要中和结构域（V3）的肽是否可以用作抗体结合试验中的抗原，以预测受试者病毒的基因型。从四个WHO申办的疫苗评价中心（乌干达、卢旺达、泰国和巴西）的HIV-1感染受试者中采集的血清样本，通过抗体与一组合成V3肽的结合进行表征，这些合成V3肽来源于根据env系统发育分析（A-E）确定的HIV-1亚组V3区共有序列。使用间接V3肽结合试验进行初步筛选，然后使用V3肽抗原限制性ELISA作为二级试验，以区分交叉反应性（如果筛选试验不确定）。一般来说，V3肽血清学可以预测HIV-1基因型。在已知病毒基因型的血清中，肽测定法可以预测大约90%的A、B、C和E基因型病例的正确基因型;基因型D的乌干达血清反应性更广泛。在某些HIV-1基因型之间，特别是A和C之间，以及在较小程度上，B和D亚型之间，存在相当大的血清学交叉反应性。由于V3环冠部的多态性，需要额外的B肽（B '）来分型巴西B基因型血清。这些简单的检测方法可能有助于确定和分布在人群中传播的HIV-1基因型。
We have investigated whether peptides representing the HIV-1 principal neutralization domain (V3) can be used as antigens in antibody-binding assays to predict the genotypes of the subjects' virus. Serum samples collected from HIV-1-infected subjects from the four WHO-sponsored vaccine evaluation sites (Uganda, Rwanda, Thailand, and Brazil) were characterized by antibody binding to a panel of synthetic V3 peptides that were derived from the consensus sequences of the V3 region of the HIV-1 subgroups according to the env phylogenetic analysis (A-E). An indirect V3 peptide-binding assay was used for primary screening, and a V3 peptide antigen-limiting ELISA was then used as a secondary assay to discriminate cross-reactivity if the screening assay was equivocal. In general, V3 peptide serology could predict HIV-1 genotypes. In sera for which the genotype of the virus was known, peptide assays could predict the correct genotype in approximately 90% of cases for genotypes A, B, C, and E; Ugandan sera of genotype D were more broadly reactive. There was considerable serological cross-reactivity between some HIV-1 genotypes, in particular between A and C, and, to a lesser extent, B and D subtypes. Owing to polymorphism at the crown of the V3 loop, an additional B peptide (B') was required to type Brazilian B genotype sera. These simple assays may help facilitate the determination and distribution of HIV-1 genotypes circulating in populations.