Radiation stability and modification of gelatin for biological and medical applications
Radiation stability and modification of gelatin for biological and medical applications
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DOI：
10.1016/j.radphyschem.2014.05.056
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发表时间：
2014-10-01
影响因子：
2.9
通讯作者：
Taguchi, Mitsumasa
中科院分区：
文献类型：
--
作者：
Haema, Kamonwon;Oyama, Tomoko Gowa;Taguchi, Mitsumasa
明胶用于各种生物和医学领域，包括药物递送系统和组织工程。在这些应用的背景下，从辐射稳定性的角度评价了明胶的辐射灭菌。采用凝胶渗透色谱法（GPC）分析了电子束辐照后明胶粉末的分子量。我们发现，辐照分解的明胶和重均摩尔质量（M-W）减少了约7-10%，灭菌剂量在5-25 kGy的范围内。此外，我们发现，在身体和细胞培养环境（37摄氏度的水）的水解率受到辐射的影响。虽然明胶粉末在辐照时发生断链，但当明胶在水溶液中辐照时主要诱导交联。在不使用任何交联剂的情况下制备辐射交联（RX）明胶水凝胶。在这种情况下，同时进行制造和辐射灭菌。使用洗脱溶胶的凝胶级分和GPC分析，确定RX-明胶水凝胶在37 ℃的水中稳定7天。这些结果为评价明胶和RX-明胶水凝胶的生物和医学应用的可行性提供了重要数据。(C)2014爱思唯尔有限公司版权所有。
Gelatin is used in various biological and medical fields, including drug delivery systems and tissue engineering. In the context of these applications, radiation sterilization of gelatin was evaluated in terms of radiation stability. The molecular weight of gelatin powder irradiated by electron beams (EB) was analyzed using gel permeation chromatography (GPC). We found that irradiation decomposed the gelatin and that the weight-averaged molar mass (M-w) decreased by approximately 7-10% with sterilization doses in the range of 5-25 kGy. Also, we found that the hydrolysis rate in body and cell culture environments (37 degrees C water) was affected by irradiation. Although gelatin powder underwent chain scission when irradiated, crosslinking was predominantly induced when the gelatin was irradiated in water solution. Radiation-crosslinked (RX) gelatin hydrogel was fabricated without using any crosslinkers. In this case, fabrication and radiation sterilization were performed simultaneously. Using gel fraction and GPC analysis of the eluted sol, it was determined that the RX-gelatin hydrogel was stable for 7 days in water at 37 degrees C. These results provide important data for evaluating the feasibility of biological and medical applications of gelatin and RX-gelatin hydrogel. (C) 2014 Elsevier Ltd. All rights reserved.