Assay for Blood-brain Barrier Integrity in Drosophila melanogaster.
Assay for Blood-brain Barrier Integrity in Drosophila melanogaster.
复制标题
果蝇血脑屏障完整性的测定。
DOI：
10.3791/60233
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Jennifer C Jemc
中科院分区：
文献类型：
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作者：
Matthew J. Davis;Danielle E. Talbot;Jennifer C Jemc
适当的神经系统发育包括血脑屏障的形成，血脑屏障是一种扩散屏障，它严格调节神经系统的通路，并保护神经组织免受毒素和病原体的侵害。这种屏障形成的缺陷与神经病变相关，并且在许多神经退行性疾病中观察到这种屏障的破坏。因此，确定调控血脑屏障形成和维持的基因以确定潜在的治疗靶点至关重要。为了了解这些基因在神经发育中的确切作用，有必要分析改变的基因表达对血脑屏障完整性的影响。许多在建立血脑屏障中起作用的分子在真核生物中是保守的，包括果蝇。果蝇已被证明是一个很好的模型系统，用于检查调节神经系统发育和功能的分子机制。本研究描述了一个逐步的程序来测定血脑屏障完整性在胚胎和幼虫阶段的D。黑腹发育
Proper nervous system development includes the formation of the blood-brain barrier, the diffusion barrier that tightly regulates access to the nervous system and protects neural tissue from toxins and pathogens. Defects in the formation of this barrier have been correlated with neuropathies, and the breakdown of this barrier has been observed in many neurodegenerative diseases. Therefore, it is critical to identify the genes that regulate the formation and maintenance of the blood-brain barrier to identify potential therapeutic targets. In order to understand the exact roles these genes play in neural development, it is necessary to assay the effects of altered gene expression on the integrity of the blood-brain barrier. Many of the molecules that function in the establishment of the blood-brain barrier have been found to be conserved across eukaryotic species, including the fruit fly, Drosophila melanogaster. Fruit flies have proven to be an excellent model system for examining the molecular mechanisms regulating nervous system development and function. This protocol describes a step-by-step procedure to assay for blood-brain barrier integrity during the embryonic and larval stages of D. melanogaster development.