Effects of bisphenol analogues on steroidogenic gene expression and hormone synthesis in H295R cells
Effects of bisphenol analogues on steroidogenic gene expression and hormone synthesis in H295R cells
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双酚类似物对 H295R 细胞类固醇基因表达和激素合成的影响
DOI：
10.1016/j.chemosphere.2015.12.081
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发表时间：
2016-03-01
期刊：
影响因子：
8.8
通讯作者：
Shao, Bing
中科院分区：
文献类型：
--
作者：
Feng, Yixing;Jiao, Zhihao;Shao, Bing
双酚A（BPA）的使用已在许多国家受到管制，因为它对人类健康有潜在的不利影响。作为限制的结果，结构类似物如双酚S（BPS）和双酚F（BPF）已经作为BPA的替代品用于工业应用。双酚AF（BPAF）主要用作特种含氟弹性体合成中的交联剂。这些化合物已在各种环境基质和人体样本中检测到。先前的研究表明，这些化合物对野生动物和哺乳动物具有潜在的内分泌干扰作用。然而，对肾上腺皮质功能的影响和潜在的机制尚未完全了解。本研究以H295 R细胞为模型，比较了BPA、BPS、BPF和BPAF四种BPs的细胞毒性，并探讨了它们的内分泌干扰作用。72 h的半致死浓度（LC 50）表明，BPAF > BPA > BPS > BPF。激素结果表明，BPA类似物，如BPF，BPS和BPAF能够改变H295 R细胞中的类固醇合成。BPA和BPS表现出抑制激素的产生，BPF主要导致孕酮和1713-雌二醇水平的增加，BPAF表现出孕酮的诱导和睾酮的减少。BPA和BPAF对H295 R细胞激素分泌的抑制作用可能是通过下调类固醇基因来介导的。然而，BPF和BPS的内分泌干扰作用的机制尚不清楚，这可能有BPA未检测到的其他机制。(C)2015爱思唯尔有限公司版权所有。
The use of Bisphenol A (BPA) has been regulated in many countries because of its potential adverse effects on human health. As a result of the restriction, structural anologues such as bisphenol S (BPS) and bisphenol F (BPF) have already been used for industrial applications as alternatives to BPA. Bisphenol AF (BPAF) is mainly used as a crosslinker in the synthesis of specialty fluoroelastomers. These compounds have been detected in various environmental matrices and human samples. Previous studies have shown that these compounds have potential endocrine disrupting effects on wildlife and mammals in general. However, the effects on adrenocortical function and the underlying mechanisms are not fully understood. In the present study, the H295R cell line was used as a model to compare the cell toxicity and to investigate the potential endocrine disrupting action of four BPs (including BPA, BPS, BPF, and BPAF). The half lethal concentration (LC50) values at 72 h exposure indicated that the rank order of toxicities of the chemicals was BPAF > BPA > BPS > BPF. The hormone results demonstrated that BPA analogues, such as BPF, BPS and BPAF were capable of altering steroidogenesis in H295R cells. BPA and BPS exhibited inhibition of hormone production, BPF predominantly led to increased progesterone and 1713-estradiol levels and BPAF showed induction of progesterone and reduction of testosterone. Inhibition effects of BPA and BPAF on hormone production were probably mediated by down-regulation of steroidogenic genes in H295R cells. However, the mechanisms of the endocrine interrupting action of BPF and BPS are still unclear, which may have additional mechanisms that have not been detected with BPA. (C) 2015 Elsevier Ltd. All rights reserved.