The cell cycle regulator GpsB functions as cytosolic adaptor for multiple cell wall enzymes
The cell cycle regulator GpsB functions as cytosolic adaptor for multiple cell wall enzymes
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DOI：
10.1038/s41467-018-08056-2
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发表时间：
2019-01-16
影响因子：
16.6
通讯作者：
Lewis, Richard J.
中科院分区：
文献类型：
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作者：
Cleverley, Robert M.;Rutter, Zoe J.;Lewis, Richard J.
细菌的生长和细胞分裂需要精确的时空调控细胞质膜周围的肽聚糖层的合成和重塑。GpsB是一种胞浆蛋白，通过与肽聚糖合成酶的细胞质微区结合来影响细胞壁的合成，以确保它们正确的亚细胞定位。在这里，我们描述了GpsB与来自三个细菌物种(枯草芽孢杆菌、单核细胞增生性李斯特菌和肺炎链球菌)的肽聚糖合成酶相互作用的关键结构特征，并指出它们对单核细胞增生性李斯特菌和肺炎链球菌的细胞壁生长和生存能力的重要性。我们使用这些结构基序来识别枯草杆菌中GpsB的新伙伴，并在所有三个细菌物种中扩展GpsB相互作用组的成员。我们的结果支持GpsB作为一种适配蛋白，介导膜蛋白、支架蛋白、信号蛋白和酶之间的相互作用，以依赖于细菌细胞周期的方式在特定位置产生更大的蛋白质复合体。
Bacterial growth and cell division requires precise spatiotemporal regulation of the synthesis and remodelling of the peptidoglycan layer that surrounds the cytoplasmic membrane. GpsB is a cytosolic protein that affects cell wall synthesis by binding cytoplasmic mini-domains of peptidoglycan synthases to ensure their correct subcellular localisation. Here, we describe critical structural features for the interaction of GpsB with peptidoglycan synthases from three bacterial species (Bacillus subtilis, Listeria monocytogenes and Streptococcus pneumoniae) and suggest their importance for cell wall growth and viability in L. monocytogenes and S. pneumoniae. We use these structural motifs to identify novel partners of GpsB in B. subtilis and extend the members of the GpsB interactome in all three bacterial species. Our results support that GpsB functions as an adaptor protein that mediates the interaction between membrane proteins, scaffolding proteins, signalling proteins and enzymes to generate larger protein complexes at specific sites in a bacterial cell cycle-dependent manner.