Yeast MEK-dependent signal transduction: response thresholds and parameters affecting fidelity.
Yeast MEK-dependent signal transduction: response thresholds and parameters affecting fidelity.
复制标题
酵母 MEK 依赖性信号转导：影响保真度的响应阈值和参数。
DOI：
10.1128/mcb.15.12.6545
复制
发表时间：
1995
影响因子：
5.3
通讯作者：
Errede,B
中科院分区：
文献类型：
--
作者：
Yashar,B;Irie,K;Printen,JA;Stevenson,BJ;SpragueJr,GF;Matsumoto,K;Errede,B
Ste7p and Mkk1p are MEK (MAPK/ERK kinase) family members that function in the mating and cell integrity signal transduction pathways inSaccharomyces cerevisiae. We selectedSTE7andMKK1mutations that stimulated their respective pathways in the absence of an inductive signal. Strikingly, serine-to-proline substitutions at analogous positions in Ste7p (position 368) and Mkk1p (position 386) were recovered by independent genetic screens. Such an outcome suggests that this substitution in other MEKs would exhibit similar properties. The Ste7p-P368variant has higher basal enzymatic activity than Ste7p but still requires induction to reach full activation. The higher activity associated with Ste7p-P368allows it to compensate for defects in the cell integrity pathway, but it does so only when it is overproduced or when Ste5p is missing. This behavior suggests that Ste5p, which has been proposed to be a tether for the kinases in the mating pathway, contributes to Ste7p specificity.
