Influence of a layer-by-layer-assembled multilayer of anti-CD34 antibody, vascular endothelial growth factor, and heparin on the endothelialization and anticoagulation of titanium surface
Influence of a layer-by-layer-assembled multilayer of anti-CD34 antibody, vascular endothelial growth factor, and heparin on the endothelialization and anticoagulation of titanium surface
复制标题
抗CD34抗体、血管内皮生长因子和肝素逐层组装多层膜对钛表面内皮化和抗凝的影响
DOI：
10.1002/jbm.a.34392
复制
发表时间：
2013-04-01
影响因子：
4.9
通讯作者：
Huang, Nan
中科院分区：
文献类型：
--
作者：
Liu, Shihui;Liu, Tao;Huang, Nan
血管支架金属表面内皮化作为一种改善血管内支架生物相容性的新方法而受到关注。本文的研究重点是利用抗cd34抗体、血管内皮生长因子（VEGF）、肝素等通过逐层自组装技术在活化钛表面构建生物功能层，促进钛表面内皮化。与钛表面相比，培养5 d后LBLs上粘附内皮祖细胞（EPCs）的数量增加了47.1%，同时培养5 d后LBLs上EPCs的增殖率也有显著提高。血液相容性评价结果清楚地表明，LBL降低了粘附和活化血小板的数量（粘附：Ti比LBL减少83%）
The endothelialization of the metal surface of vascular stents came into focus as a new method for improving the biocompatibility of intravascular stents. This article has its focus on building a biofunctional layer on the activated titanium surface with anti-CD34 antibody, vascular endothelial growth factor (VEGF), and heparin by a layer-by-layer (LBL) self-assembly technique, to promote the endothelialization of the surface. When compared with titanium surface, the number of adhered endothelial progenitor cells (EPCs) on LBLs increased 47.1% after 5 days culture, meanwhile the proliferation rate of EPCs on LBLs during 5 days culture also exhibit significantly improvement. The results of blood compatibility evaluation clearly show that the LBLs reduce the number of adhered and activated blood platelets (adhered: Ti 83% vs. LBL