The effect of emodin on melanogenesis through the modulation of ERK and MITF signaling pathway
The effect of emodin on melanogenesis through the modulation of ERK and MITF signaling pathway
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DOI：
10.1080/14786419.2020.1849200
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发表时间：
2020-11-10
影响因子：
2.2
通讯作者：
Kim, Moon-Moo
中科院分区：
文献类型：
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作者：
Kim, Jaeho;Kim, Moon-Moo
本研究旨在探讨何首乌大黄素对B16F1细胞黑素生成的影响。在本研究中，大黄素在DPPH自由基和还原能力测定中没有显示出抗氧化活性。然而，大黄素被发现清除细胞内的过氧化氢。大黄素在体外不仅能增加酪氨酸酶活性，还能增加黑色素的合成。免疫印迹和免疫荧光分析显示，与过氧化氢处理组相比，大黄素可通过上调酪氨酸酶(TYR)、酪氨酸相关蛋白(Trp)-1、Trp-2、MITF和SIRT1蛋白的表达水平来促进黑素合成。此外，大黄素抑制SIRT1和FOXO1激活的ERK。因此，大黄素通过激活MITF转录因子，增加TRP-1、TRP-2、酪氨酸酶的表达，从而促进黑色素的合成。这些发现表明，大黄素可以促进黑色素的产生，这与防止头发变白有关。
The aim of this study was to investigate the effect of emodin derived from Polygonum multiflorum on melanin production in B16F1 cells. In this study, emodin did not show antioxidant activity in DPPH radical and reducing power assays. However, it was found that emodin scavenged intracellular H2O2. Emodin increased not only tyrosinase activity but also melanin synthesis in vitro. Moreover, emodin enhanced melanin synthesis by increasing the expression level of tyrosinase (TYR), tyrosine related protein (TRP)-1, TRP-2, MITF and SIRT1 proteins in live cells treated with H2O2 compared with H2O2 treatment group in the analyses of western blot and immunofluorescence. Moreover, emodin suppressed ERK activation by SIRT1 and FOXO1. Thus, emodin promoted melanin synthesis by increasing the expression of TRP-1, TRP-2, tyrosinase through the activation of MITF transcription factor. These findings suggest that emodin could promote melanin production related to anti-hair graying.