Conserved Activity of Reassociated Homotetrameric Protein Subunits Released from Mesoporous Silica Nanoparticles
Conserved Activity of Reassociated Homotetrameric Protein Subunits Released from Mesoporous Silica Nanoparticles
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DOI：
10.1021/acs.langmuir.7b03310
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发表时间：
2018-01-09
期刊：
影响因子：
3.9
通讯作者：
Trewyn, Brian G.
中科院分区：
文献类型：
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作者：
Deodhar, Gauri V.;Adams, Marisa L.;Trewyn, Brian G.
制备并表征了具有大孔结构的介孔二氧化硅纳米颗粒(MSN)，通过多种生化和材料表征，可逆地将刀豆蛋白A亚单位包埋在介孔中。当装载到MSN中时，我们展示了来自蛋白酶的蛋白质稳定性，当释放时，通过甘露糖结合和邻苯二甲醛荧光显示亚基重新结合成活性蛋白质。我们已经展示了一种通用和简便的方法来将同源蛋白装载到MSN中，并在增强大的治疗性蛋白的输送方面具有潜在的应用。
Mesoporous silica nanoparticles (MSN) with enlarged pores were prepared and characterized, and reversibly dissociated subunits of concanavalin A were entrapped in the mesopores, as shown by multiple biochemical and material characterizations. When loaded in the MSN, we demonstrated protein stability from proteases and, upon release, the subunits reassociated into active proteins shown through mannose binding and o-phthalaldehyde fluorescence. We have demonstrated a versatile and facile method to load homomeric proteins into MSN with potential applications in enhancing the delivery of large therapeutic proteins.