Fgf4 positively regulates scleraxis and tenascin expression in chick limb tendons
Fgf4 positively regulates scleraxis and tenascin expression in chick limb tendons
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DOI：
10.1006/dbio.2002.0707
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发表时间：
2002-07-15
影响因子：
2.7
通讯作者：
Duprez, D
中科院分区：
文献类型：
--
作者：
Edom-Vovard, F;Schuler, B;Duprez, D
在脊椎动物中，肌腱将肌肉与骨骼连接起来。小鸡的外科手术实验强调了肌腱和肌肉之间的发育相互作用。肌腱的初始形成相对于肌肉自主地发生。然而，进一步的肌腱发育需要肌肉的存在。参与这些相互作用的分子信号仍然未知。在小鸡的肢体中，Fgf4转录本位于肌肉的末端，未来的肌腱将附着在那里。在本文中，我们分析了肌肉Fgf4对肌腱发育的假定作用。我们已经使用了三个一般的肌腱标记物，scleraxis，tenascin，和FGF8来分析FGF4在不同实验条件下对这些肌腱相关分子的调节。在缺乏Fgf4的情况下，在无肌肉和无神经的肢体中，三种肌腱相关分子，即硬化蛋白、腱生蛋白和Fgf8的表达下调。在正常、无神经和无肌肉的肢体中外源性植入FGF4可诱导硬化和腱生蛋白表达，但不诱导FGF8表达。这些结果表明，肌肉中表达的Fgf4是维持巩膜轴和腱生蛋白所必需的，而肌腱中不表达Fgf8。(C)2002 Elsevier Science（美国）。
In vertebrates, tendons connect muscles to skeletal elements. Surgical experiments in the chick have underlined developmental interactions between tendons and muscles. Initial formation of tendons occurs autonomously with respect to muscle. However, further tendon development requires the presence of muscle. The molecular signals involved in these interactions remain unknown. In the chick limb, Fgf4 transcripts are located at the extremities of muscles, where the future tendons will attach. In this paper, we analyse the putative role of muscle-Fgf4 on tendon development. We have used three general tendon markers, scleraxis, tenascin, and Fgf8 to analyse the regulation of these tendon-associated molecules by Fgf4 under different experimental conditions. In the absence of Fgf4, in muscleless and aneural limbs, the expression of the three tendon-associated molecules, scleraxis, tenascin, and Fgf8, is down-regulated. Exogenous implantation of Fgf4 in normal, aneural, and muscleless limbs induces scleraxis and tenascin expression but not that of Fgf8. These results indicate that Fgf4 expressed in muscle is required for the maintenance of scleraxis and tenascin but not Fgf8 expression in tendons. (C) 2002 Elsevier Science (USA).