MAPPING THE DISTRIBUTION OF THE OUTER HAIR CELL MOTILITY VOLTAGE SENSOR BY ELECTRICAL AMPUTATION
MAPPING THE DISTRIBUTION OF THE OUTER HAIR CELL MOTILITY VOLTAGE SENSOR BY ELECTRICAL AMPUTATION
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DOI：
10.1016/s0006-3495(93)81248-7
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发表时间：
1993-11-01
影响因子：
3.4
通讯作者：
SANTOS-SACCHI, J
中科院分区：
文献类型：
--
作者：
HUANG, GJ;SANTOS-SACCHI, J
外毛细胞(OHC)具有非线性电荷运动，其特征表明它代表负责OHC机械活动的电压传感器。OHC的机械活动已知存在于细胞长度的有限范围内。我们使用了同时分区微室和全电池电压钳技术来电隔离OHC膜的部分，发现非线性电荷运动沿电池的长度也受到限制。OHC的顶端和底部没有电压传感器，对应于细胞的亚表层脑池和/或机械反应缺失的区域。我们得出结论，运动电压传感器的物理域对应于机械效应器的物理域，并推测传感器和效应器驻留在一个膜内分子物种中，可能是进化的非导电或弱导电的电压依赖离子通道。
The outer hair cell (OHC) possesses a nonlinear charge movement whose characteristics indicate that it represents the voltage sensor responsible for OHC mechanical activity. OHC mechanical activity is known to exist along a restricted extent of the cell's length. We have used a simultaneous partitioning microchamber and whole cell voltage clamp technique to electrically isolate sections of the OHC membrane and find that the nonlinear charge movement is also restricted along the cell's length. Apical and basal portions of the OHC are devoid of voltage sensors, corresponding to regions of the cell where the subsurface cisternae and/or the mechanical responses are absent. We conclude that the physical domain of the motility voltage sensor corresponds to that of the mechanical effector and speculate that sensor and effector reside within one intra membranous molecular species, perhaps an evolved nonconducting or poorly conducting voltage-dependent ion channel.