CIPK-B is essential for salt stress signalling in Marchantia polymorpha
CIPK-B is essential for salt stress signalling in Marchantia polymorpha
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DOI：
10.1101/2022.08.22.504506
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发表时间：
2022-08
期刊：
影响因子：
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通讯作者：
Connor Tansley;James Houghton;Althea M E Rose;Bartosz Witek;Rocky D Payet;Taoyang Wu;J. B. Miller
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文献类型：
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作者：
Connor Tansley;James Houghton;Althea M E Rose;Bartosz Witek;Rocky D Payet;Taoyang Wu;J. B. Miller
钙信号是许多植物过程的中心，钙解码蛋白家族已经扩展到绿色谱系和存在于解码之间的冗余。地钱已迅速成为一种新的模式植物，但目前尚不清楚该物种中存在哪些钙解码器。我们进行了系统发育分析，以确定多态分枝杆菌的类钙调神经磷酸酶(CBL)和CBL相互作用蛋白激酶(CIPK)网络。我们分析了CBL-CIPK在盐胁迫下的表达，并利用酵母双杂交和双分子荧光互补技术确定了蛋白质之间的相互作用。我们还使用CRISPR/Cas9创建了基因敲除。我们证实，多态分枝杆菌具有两个CIPK和三个CBL。两个CIPK和只有一个CBL显示出盐响应的转录变化。在植物中，所有的多态分枝杆菌CBL-CIPK相互作用。敲除CIPK-B导致对盐的敏感性增加，这表明该CIPK参与了盐信号转导。我们已经鉴定了CBL-CIPKs，它们构成了多态木霉耐盐途径的一部分。系统发育和相互作用研究表明，这些CBL-CIPK形成了一条进化保守的盐过度敏感(SOS)途径。因此，盐反应可能是CBL-CIPK网络的一些早期功能，并增加了陆地植物出苗所需的非生物胁迫耐受性。
Calcium signalling is central to many plant processes, with families of calcium decoder proteins having expanded across the green lineage and redundancy existing between decoders. The liverwort Marchantia polymorpha has fast become a new model plant, but it is unclear what calcium decoders exist in this species. We have performed phylogenetic analyses to identify the Calcineurin B-Like (CBL) and CBL-Interacting Protein Kinase (CIPK) network of M. polymorpha. We analysed CBL-CIPK expression during salt stress, and determined protein-protein interactions using yeast two-hybrid and bimolecular fluorescence complementation. We also created genetic knockouts using CRISPR/Cas9. We confirm that M. polymorpha has two CIPKs and three CBLs. Both CIPKs and only one CBL show salt-responsive transcriptional changes. All M. polymorpha CBL-CIPKs interact with each other in planta. Knocking out CIPK-B causes increased sensitivity to salt suggesting that this CIPK is involved in salt signalling. We have identified CBL-CIPKs that form part of a salt tolerance pathway in M. polymorpha. Phylogeny and interaction studies imply that these CBL-CIPKs form an evolutionarily conserved Salt Overly Sensitive (SOS) pathway. Hence, salt responses may be some of the early functions of CBL-CIPK networks and increased abiotic stress tolerance required for land plant emergence.