Clustering of surface NMDA receptors is mainly mediated by the C-terminus of GluN2A in cultured rat hippocampal neurons
Clustering of surface NMDA receptors is mainly mediated by the C-terminus of GluN2A in cultured rat hippocampal neurons
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培养的大鼠海马神经元表面 NMDA 受体的聚集主要由 GluN2A C 端介导
DOI：
10.1007/s12264-014-1450-8
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发表时间：
2014-07
影响因子：
5.6
通讯作者：
Wang, Wei
中科院分区：
文献类型：
--
作者：
Xu, Shu-Jun;Luo, Jian-Hong;Qiu, Shuang;Wang, Wei
含有不同GluN 2亚基的N-甲基-D-天冬氨酸受体（NMDARs）在突触可塑性中发挥不同的作用。这种差异不仅取决于通道特性，还取决于不同的表面分布和突触定位。在本研究中，使用Cy 3-缀合的GFP抗体的Fab片段标记的表面定位的GluN 2亚基与GFP在N-末端标记，我们观察了膜分布模式的GluN 2A-或GluN 2B-含有NMDAR在培养的大鼠海马神经元。我们发现，表面NMDAR含有GluN 2A，但不是那些含有GluN 2B，倾向于集群在DIV 7。交换GluN 2亚基的羧基末端完全逆转了这些分布模式。此外，含有GluN 2A的表面NMDAR优先与PSD-95相关。综上所述，我们的研究结果表明，含GluN 2A的NMDARs的聚集分布是由GluN 2A的C-末端决定的，其与PSD-95的相互作用在这个过程中起着重要的作用。
N-methyl-D-aspartate receptors (NMDARs) containing different GluN2 subunits play distinct roles in synaptic plasticity. Such differences may not only be determined by the channel properties, but also by differential surface distribution and synaptic localization. In the present study, using a Cy3-conjugated Fab fragment of the GFP antibody to label surface-located GluN2 subunits tagged with GFP at the N-terminus, we observed the membrane distribution patterns of GluN2A- or GluN2B-containing NMDARs in cultured rat hippocampal neurons. We found that surface NMDARs containing GluN2A, but not those containing GluN2B, were inclined to cluster at DIV7. Swapping the carboxyl termini of the GluN2 subunits completely reversed these distribution patterns. In addition, surface NMDARs containing GluN2A were preferentially associated with PSD-95. Taken together, the results of our study suggest that the clustering distribution of GluN2A-containing NMDARs is determined by the GluN2A C-terminus, and its interaction with PSD-95 plays an important role in this process.
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影响因子：
16.2
作者：
Hayashi, Takashi;Thomas, Gareth M.;Huganir, Richard L.
通讯作者：
Huganir, Richard L.
影响因子：
2.5
作者：
Thomas, CG;Miller, AJ;Westbrook, GL
通讯作者：
Westbrook, GL
影响因子：
2.5
作者：
Vicini, S;Wang, JF;Grayson, DR
通讯作者：
Grayson, DR
影响因子：
4.8
作者：
Qiu, S;Hua, YL;Luo, JH
通讯作者：
Luo, JH
DOI：
10.1523/jneurosci.16-07-02157.1996
发表时间：
1996-04
期刊：
--
影响因子：
--
作者：
Martin Niethammer;Eunjoon Kim;Morgan Sheng
通讯作者：
Martin Niethammer;Eunjoon Kim;Morgan Sheng