Polylysine-functionalised thermoresponsive chitosan hydrogel for neural tissue engineering
Polylysine-functionalised thermoresponsive chitosan hydrogel for neural tissue engineering
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DOI：
10.1016/j.biomaterials.2006.08.044
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发表时间：
2007-01-01
期刊：
影响因子：
14
通讯作者：
Forsythe, J. S.
中科院分区：
文献类型：
--
作者：
Crompton, K. E.;Goud, J. D.;Forsythe, J. S.
胎鼠皮层细胞培养的2D电影和3D热响应壳聚糖/甘油磷酸盐（GP）水凝胶。根据细胞数量和每个细胞的神经突来评估壳聚糖/GP 2D膜的生物相容性。水凝胶的渗透压是促进细胞存活的关键因素，等渗GP浓度提供了最佳条件。为了提高细胞粘附和神经突起的生长，聚-D-赖氨酸（PDL）固定到壳聚糖通过叠氮苯胺光耦合。PDL浓度的增加并没有改变2D培养中的细胞存活率，但神经突生长受到显著抑制。在三维培养条件下，PDL贴壁对细胞数量、细胞形态和突起生长的影响更为明显。神经元表现出更大的细胞体，并在大孔凝胶中发出单个神经突。固定化PDL提高细胞存活至最佳浓度0.1%，然而，进一步增加导致细胞数量和神经突生长下降。这归因于与相应的2D表面相比，3D水凝胶内与PDL的细胞相互作用更高。结果表明，热响应性壳聚糖/GP水凝胶为神经组织工程提供了合适的三维支架环境。(c)2006爱思唯尔有限公司保留所有权利。
Foetal mouse cortical cells were cultured on 2D films and within 3D thermally responsive chitosan/glycerophosphate salt (GP) hydrogels. The biocompatibility of chitosan/GP 2D films was assessed in terms of cell number and neurites per cell. Osmolarity of the hydrogel was a critical factor in promoting cell survival with isotonic GP concentrations providing optimal conditions. To improve cell adhesion and neurite outgrowth, poly-D-lysine (PDL) was immobilised onto chitosan via azidoaniline photocoupling. Increase in PDL concentrations did not alter cell survival in 2D cultures but neurite outgrowth was significantly inhibited. Neurons exhibited a star-like morphology typical of 2D culture systems.The effects of PDL attachment on cell number, cell morphology and neurite outgrowth were more distinct in 3D culture conditions. Neurones exhibited larger cell bodies and sent out single neurites within the macroporous gel. Immobilised PDL improved cell survival up to an optimum concentration of 0.1%, however, further increases resulted in drops in cell number and neurite outgrowth. This was attributed to a higher cell interaction with PDL within a 3D hydrogel compared to the corresponding 2D surface. The results show that thermally responsive chitosan/GP hydrogels provide a suitable 3D scaffolding environment for neural tissue engineering. (c) 2006 Elsevier Ltd. All rights reserved.