Cytoplasmic strains and strain rates in motile polymorphonuclear leukocytes.
Cytoplasmic strains and strain rates in motile polymorphonuclear leukocytes.
复制标题
运动多形核白细胞的细胞质应变和应变率。
DOI：
10.1016/s0006-3495(90)82379-1
复制
发表时间：
1990
影响因子：
3.4
通讯作者：
Schmid-Schonbein,GW
中科院分区：
文献类型：
--
作者：
Simon,SI;Schmid-Schonbein,GW
本文提出了一种新的测量活动性中性粒细胞胞质局部变形和变形率的方法。变形用生物力学应变和应变率表示。为此目的，使用小的吞噬乳胶微球作为细胞内标记物。平面拉格朗日和欧拉应变和应变率估计从一个三联体的内化标记的位置。计算了主应变、拉伸比和主方向。发现细胞内应变相对于整体细胞形状变化较大。主要的细胞质拉伸比表现出大的扩展方向的伪足形成和细胞运动和收缩在垂直方向。区域应变分析表明，收缩应变占主导地位的伪足或运动的前缘附近。伪足和主细胞体之间的过渡区表现出较大的剪切应变。后区，尾足位于，也显示了大的扩展，但小的收缩应变。应变率相对较小，时间上不均匀，并且在很大程度上与应变无关。我们提出的测量细胞质应变的方法可以应用于细胞力学中的各种问题。
A new method is presented to measure local cytoplasmic deformation and rate of deformation in motile active neutrophils. The deformation is expressed in terms of biomechanical strains and strain rates. For this purpose small phagocytosed latex microspheres were used as intracellular markers. Planar Lagrangian and Eulerian strains and the rate of strain were estimated from the positions of a triad of internalized markers. Principal strains, stretch ratios, and principal directions were computed. The intracellular strains were found to be large relative to the overall cell shape change. Principal cytoplasmic stretch ratios showed large extension in the direction of pseudopod formation and cell locomotion and contraction in perpendicular directions. Regional strain analysis showed contractile strains to predominate in the vicinity of the pseudopod or leading edge of motion. The transitional region between the pseudopod and the main cell body exhibited large shear strains. The posterior region, where the uropod is located, also revealed large extensions but small contractile strains. The rate of strains are relatively small, nonuniform in time, and largely independent of the strain. The method we propose to measure cytoplasmic strain can be applied to a variety of problems in cell mechanics.