EXPRESSION OF DESMIN, LAMININ AND FIBRONECTIN DURING INSITU DIFFERENTIATION (DECIDUALIZATION) OF RAT UTERINE STROMAL CELLS
EXPRESSION OF DESMIN, LAMININ AND FIBRONECTIN DURING INSITU DIFFERENTIATION (DECIDUALIZATION) OF RAT UTERINE STROMAL CELLS
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DOI：
10.1111/j.1432-0436.1987.tb00161.x
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发表时间：
1987-01-01
期刊：
影响因子：
2.9
通讯作者：
JULIAN, J
中科院分区：
文献类型：
--
作者：
GLASSER, SR;LAMPELO, S;JULIAN, J
含有蜕膜组织的冷冻大鼠子宫切片的免疫细胞化学分析表明，蜕膜细胞中中间丝蛋白结蛋白的表达升高，所述蜕膜组织形成于对由内源性或外源性激素方案致敏的子宫的正常或人工刺激的响应。细胞外基质（ECM）成分表达的变化与间质细胞进行蜕膜化时结蛋白表达的升高相协调。与区域蜕膜化的模式平行，由升高的结蛋白表达确定，层粘连蛋白在蜕膜细胞ECM中积累，而纤连蛋白明显减少与蜕膜细胞表面的组织改变相关。原位观察证实了先前的结果，这表明结蛋白在体内或体外形成的蜕膜细胞中的表达是其分化的有效标志物，并解决了先前研究中未回答的问题：（a）结蛋白（和层粘连蛋白）似乎在非蜕膜化基质中以几乎检测不到的水平组成性表达，（B）结蛋白是基质细胞分化的有效标志物，因为它在蜕膜细胞中类似地表达，而与子宫敏化和刺激的不同实验方案无关，和（c）结蛋白表达遵循与形态学和组织化学研究中描述的蜕膜化过程相同的区域进展。
Immunocytochemical analysis of frozen rat uterine sections containing decidual tissue, formed in response to normal or artificial stimulation of uteri sensitized by endogenous or exogenous hormonal regimens, demonstrated an elevated expression of the intermediate filament protein desmin in decidual cells. Changes in the expression of extracellular matrix (ECM) components were coordinated with the elevated expression of desmin as stromal cells underwent decidualization. In parallel with the pattern of regional decidualization, as determined by elevated desmin expression, laminin accumulated in ECM of decidual cells while an apparent decrease in fibronectin was associated with altered organization at the decidual cell surface. The in situ observations confirm previous results, which indicated that the expression of desmin in decidual cells formed in vivo or in vitro is a valid marker of their differentiation, and resolve questions unanswered in the previous study: (a) desmin (and laminin) appear to be constitutively expressed in non-decidualized stroma at barely detectable levels, (b) desmin is a valid marker of stromal cell differentiation because it is expressed similarly in decidual cells, irrespective of varying experimental protocols for uterine sensitization and stimulation, and (c) desmin expression follows the same regional progression described for the process of decidualization in morphological and histochemical studies.