Functional characterization of two different Kupffer cell populations of normal rat liver
Functional characterization of two different Kupffer cell populations of normal rat liver
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DOI：
10.1016/s0168-8278(96)80212-1
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发表时间：
1996-10-01
影响因子：
25.7
通讯作者：
Ramadori, G
中科院分区：
文献类型：
--
作者：
Armbrust, T;Ramadori, G
背景：肝脏枯否细胞是组织巨噬细胞中最大的群体。正常肝脏中的小和大枯否细胞是不同的，这表明它们具有不同的功能。本研究旨在进一步研究正常大鼠肝脏的小和大Kupffer细胞在体内和体外的特征。方法：用单克隆抗体ED 1和ED 2双染色免疫荧光法研究大鼠肝脏切片。分离的非实质肝细胞根据大小分离，以获得小和大的枯否细胞。在培养中，吞噬作用进行了研究酵母多糖摄入和细胞增殖的H-3-胸苷掺入。结果：ED 1（+）、ED 2（+）Kupffer细胞分布于肝血窦沿着，ED 1（+）、ED 2（-）细胞主要分布于中央静脉和门静脉周围。通过逆流洗脱，将小的ED 1（+）ED 2（-）细胞与较大的ED 1（+）ED 2（+）细胞分离并培养。较大的细胞大量合成C1-抑制剂和载脂蛋白E，而小细胞仅合成痕量的这些蛋白质。干扰素-γ增加C1-抑制剂的合成在小（5倍）和大细胞（1.5倍），H-3-胸苷掺入是11倍，在小细胞比在大细胞。然而，脂多糖诱导的前白细胞介素-1 α和前白细胞介素-1 β的合成和吞噬活性在两个population.Conclusions相似：数据表明两个不同的人口单核吞噬细胞在正常大鼠肝脏以及区分免疫细胞化学和功能标记。
Background: Kupffer cells of the liver represent the largest population of tissue macrophages. Small and large Kupffer cells were distinguished in normal liver, leading to the suggestion that they have different functions. This study intends to further characterize small and large Kupffer cells of normal rat liver in vivo and in vitro.Methods: Sections of rat liver were in investigated by double-staining immunofluorescence with the monoclonal antibodies ED1 and ED2. Isolated nonparenchymal liver cells mere separated according to size to obtain small and large Kupffer cells. In culture, phagocytosis was studied by zymosan ingestion and cell proliferation by incorporation of H-3-thymidine. Synthesis of the proteins C1-inhibitor, apolipoprotein E and interleukin-1 was studied by endogenous labeling of newly synthesized proteins, immunoprecipitation and sodium dodecylsulfate-polyacrylamide gel electrophoresis.Results: ED1(+) ED2(+) Kupffer cells were located in the liver along the sinusoids, ED1(+) ED2(-) cells were found mainly located around the central vein and portal vessels. By counterflow elution, small ED1(+) ED2(-) cells were separated from larger ED1(+) ED2(+) cells and cultured. The larger cells abundantly synthesized C1-inhibitor and apolipoprotein E, while the small cells synthesized only trace amounts of these proteins. Interferon-gamma increased C1-inhibitor synthesis in small (5-fold) and large cells (1.5-fold), H-3-thymidine incorporation was 11-fold higher in small than in large cells. However, lipopolysaccharide-induced pro-interleukin-1 alpha and pro-interleukin-1 beta synthesis and phagocytic activity were similar in both populations.Conclusions: The data demonstrate two different populations of mononuclear phagocytes in normal rat liver well distinguished by immunocytochemical and functional markers.