Investigation of the spread of Brucella canis via the US interstate dog trade
Investigation of the spread of Brucella canis via the US interstate dog trade
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DOI：
10.1016/j.ijid.2006.12.009
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发表时间：
2007-09-01
影响因子：
8.4
通讯作者：
Kurth, Kathy
中科院分区：
文献类型：
--
作者：
Brower, Alexandra;Okwumabua, Ogi;Kurth, Kathy
目的：这项研究的目的是说明和帮助解决日益增长的需要监管或分子工具，以跟踪和控制犬布鲁氏菌病的传播。我们的研究目标是首先描述在美国犬只贸易背景下威斯康星州犬舍爆发的犬种布鲁氏菌，然后确定一种可能用于B菌株鉴别的分子技术。方法：威斯康星州兽医诊断实验室（WVDL）B.回顾了1995 ~ 2005年的犬布鲁氏菌病血清学资料，对威斯康星州犬舍发生的3起犬布鲁氏菌病疫情进行了调查，并对8起B.对威斯康星州、密苏里州和阿肯色州犬舍分离的犬种进行核糖体分型、脉冲场凝胶电泳（PFGE）、外膜蛋白分析（OMPA）和细胞脂肪酸富集（CFAP）。来自威斯康星州犬舍的犬阳性样本，以及威斯康星州的爆发与州际狗贸易有关。所有的B。我们检测的犬分离株在遗传上是同源的，因此不能通过核糖分型、PFGE和OMPA来区分。CFAP甲基酯分析具有一定的区分力，但聚类分析结果将菌株分为两类，说明CFAP甲基酯分析具有一定的区分力。用于B的流行病学追踪。犬的爆发，并将是有用的比较研究，作为分离的B。canis继续在全球扩张。(C)2007年国际传染病学会。由爱思唯尔有限公司出版。保留所有权利。
Objectives: The aim of this study was to illustrate and help address a growing need for regulatory or molecular tools to track and control the spread of canine brucellosis. Our study objectives were to first characterize Brucella canis outbreaks in Wisconsin kennels in the context of the dog trade in the USA, and then to identify a molecular technique that may be useful for strain differentiation of B. canis isolates.Methods: Wisconsin Veterinary Diagnostic Laboratory (WVDL) B. canis serology data from 1995 to 2005 were reviewed, three canine brucellosis outbreaks in Wisconsin dog kennels were investigated, and eight B. canis isolates recovered from Wisconsin outbreaks and kennels in Missouri and Arkansas and four isolates received from outside sources were subjected to ribotyping, pulsed-field get electrophoresis (PFGE), outer membrane protein analysis (OMPA), and cellular fatty acid profiting (CFAP).Results: WVDL has received increasing numbers of B. canis positive samples from Wisconsin kennels, and Wisconsin outbreaks are associated with the interstate dog trade. All of the B. canis isolates we examined were genetically homogenous and as such could not be differentiated by ribotyping, PFGE and OMPA. However, dendrogram analysis of CFAP divided the isolates into two groups, indicating that CFAP methyl ester analysis has discriminatory power.Conclusions: CFAP methyl ester analysis has promise as a tool. for epidemiological tracing of B. canis outbreaks and will be useful in comparison studies as isolation of B. canis continues to expand globally. (C) 2007 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.