The rice WUSCHEL-related homeobox genes are involved in reproductive organ development, hormone signaling and abiotic stress response
The rice WUSCHEL-related homeobox genes are involved in reproductive organ development, hormone signaling and abiotic stress response
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水稻 WUSCHEL 相关同源盒基因参与生殖器官发育、激素信号传导和非生物胁迫反应
DOI：
10.1016/j.gene.2014.08.003
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发表时间：
2014-10-10
期刊：
影响因子：
3.5
通讯作者：
Zhao, Yu
中科院分区：
文献类型：
--
作者：
Cheng, Saifeng;Huang, Yulan;Zhao, Yu
WUSCHEL相关同源框基因（WOX）是参与植物发育过程的重要转录调控基因。水稻（Oryzasativa L）基因组编码至少13个WOX成员。本研究利用微阵列技术对11个WOX基因在籼稻品种MH 63的8个生殖器官和8个营养器官的16个组织器官中的表达进行了系统分析。结果表明，4个基因（OsWUS、OsNS 1/Os11.52、OsWOX 3和OsWOX 9A）在幼穗和胚乳发育中特异表达，6个基因（O 5 WOX 5、OsWOX 9 B、OsWOX 9D、OsWOX 11、OsWOX 12 A和O 5 WOX 12 B）在种子中（吸胀后72 h）在根萌发或生长过程中特异表达。原位杂交分析表明OsWOX 4、OsWOX 5、OsWOX 9A和OsWOX 12 B在幼穗发育和胚胎发生过程中的转录水平存在差异。qRT-PCR结果表明，OsWOX 5、OsWOX 11、OsWOX 12 B和OsWOX 12 A这4个水稻WOX基因的表达受植物激素（生长素、细胞分裂素和赤霉素）的上调或下调。更有趣的是，大多数WOX基因对干旱、盐和寒冷等非生物胁迫刺激有反应。本研究为进一步了解水稻WOX基因家族在水稻发育、激素信号转导和非生物胁迫应答中的功能提供了理论依据。(C)2014爱思唯尔有限公司版权所有。
The WUSCHEL-related homeobox (WOX) genes are important transcription regulators participated in plant development processes. Rice (Oryzasativa L) genome encodes at least 13 WOX members. In this study, a systematic microarray-based gene expression profiling of eleven WOX genes was performed for the whole life cycle of rice at 16 different tissues/organs of MH63 (rice indica cultivar), which included eight reproductive organs and eight vegetative tissues. The results demonstrated that four genes (OsWUS, OsNS1/Os11.52, OsWOX3 and OsWOX9A) were specifically expressed in panicle and endosperm development, and six genes (O5WOX5, OsWOX9B, OsWOX9D,OsWOX11,OsWOX12A and O5WOX12B) were preferentially expressed in seeds (72 h after imbibitions) during root emergence or growth. In situ hybridization analysis revealed differential transcript levels of OsWOX4, OsWOX5, OsWOX9A and OsWOX12B during panicle development and embryogenesis. Results of qRT-PCR showed that expression of four rice WOX genes (OsWOX5, OsWOX11, OsWOX12B and OsWOX12A) was up- or down-regulated by plant hormones (auxin, cytokinin and gibberellin). More interestingly, most WOX genes were responsive to abiotic stress stimuli of drought, salt and cold. The molecular studies presented here will further provide insight in understanding the functions of rice WOX gene family in rice development, hormone signaling, and abiotic stress response. (C) 2014 Elsevier B.V. All rights reserved.