Evidence for the dissolution of molybdenum during tribocorrosion of CoCrMo hip implants in the presence of serum protein.
Evidence for the dissolution of molybdenum during tribocorrosion of CoCrMo hip implants in the presence of serum protein.
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DOI：
10.1016/j.actbio.2016.08.051
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发表时间：
2016-11
影响因子：
9.7
通讯作者：
T. A. Simões;M. Bryant;Andy P. Brown;S. J. Milne;M. Ryan;A. Neville;R. Brydson
中科院分区：
文献类型：
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作者：
T. A. Simões;M. Bryant;Andy P. Brown;S. J. Milne;M. Ryan;A. Neville;R. Brydson
我们对 CoCrMo、金属对金属 (MoM) 植入物、磨损碎片颗粒及其在髋关节模拟器中循环后的溶解进行了表征，并将结果与在代表人体环境的溶液中研磨 CoCrMo 粉末所产生的合成磨损碎片的摩擦腐蚀联系起来。重要的是，我们采用了改进的 ICP-MS 样品制备程序来测量不同介质中磨损模拟过程中 CoCrMo 合金的离子释放情况；这涉及使用纳米多孔超滤器，可以将颗粒与溶液中的自由离子和复合物完全分离。因此，我们对 CoCrMo 植入物中金属离子的释放和金属络合物的形成提出了新的视角。新方法能够确定髋关节模拟器摩擦腐蚀过程中离子相对于复合物和颗粒的质量平衡。已测量到相对于钴和铬，钼离子的释放量要高得多。牛血清白蛋白（BSA）的存在增强了钼的溶解，这可能是由于金属-蛋白质复合物的形成。总的来说，我们相信这些结果可能对髋关节置换术患者提取液体中金属离子水平的分析和解释具有重要意义；我们建议使用此处描述的方案对这些液体中的金属水平（包括钼）进行分析。意义声明我们开发了一种重要的新方案，用于分析从髋关节植入患者和髋关节模拟器中提取的液体中的金属离子水平。利用这一过程，我们提出了关于 CoCrMo 合金植入物释放金属离子的新视角，揭示了髋关节模拟器摩擦腐蚀过程中金属离子释放水平明显低于之前的预期，并且相对于钴和铬释放了更高百分比的钼离子。无论是从基础科学还是金属-蛋白质相互作用研究的角度来看，这项工作都是相关的，有助于理解与生物摩擦腐蚀相关的持续问题以及与由 CoCrMo 合金制成的金属对金属 (MoM) 髋关节植入物相关的炎症的联系。
We have characterized CoCrMo, Metal-on-Metal (MoM) implant, wear debris particles and their dissolution following cycling in a hip simulator, and have related the results to the tribocorrosion of synthetic wear debris produced by milling CoCrMo powders in solutions representative of environments in the human body. Importantly, we have employed a modified ICP-MS sample preparation procedure to measure the release of ions from CoCrMo alloys during wear simulation in different media; this involved use of nano-porous ultrafilters which allowed complete separation of particles from free ions and complexes in solution. As a result, we present a new perspective on the release of metal ions and formation of metal complexes from CoCrMo implants. The new methodology enables the mass balance of ions relative to complexes and particles during tribocorrosion in hip simulators to be determined. A much higher release of molybdenum ions relative to cobalt and chromium has been measured. The molybdenum dissolution was enhanced by the presence of bovine serum albumin (BSA), possibly due to the formation of metal-protein complexes. Overall, we believe that the results could have significant implications for the analysis and interpretation of metal ion levels in fluids extracted from hip arthroplasty patients; we suggest that metal levels, including molybdenum, be analysed in these fluids using the protocol described here.Statement of SignificanceWe have developed an important new protocol for the analysis of metal ion levels in fluids extracted from hip implant patients and also hip simulators. Using this procedure, we present a new perspective on the release of metal ions from CoCrMo alloy implants, revealing significantly lower levels of metal ion release during tribocorrosion in hip simulators than previously thought, combined with the release of much higher percentages of molybdenum ions relative to cobalt and chromium. This work is of relevance, both from the perspective of the fundamental science and study of metal-protein interactions, enabling understanding of the ongoing problem associated with the biotribocorrosion and the link to inflammation associated with Metal-on-Metal (MoM) hip implants made from CoCrMo alloys.