Phospholipase D signaling is essential for meiosis
Phospholipase D signaling is essential for meiosis
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DOI：
10.1073/pnas.92.26.12151
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发表时间：
1995-12-19
影响因子：
11.1
通讯作者：
Engebrecht, JA
中科院分区：
文献类型：
--
作者：
Rose, K;Rudge, SA;Engebrecht, JA
磷脂代谢通过产生信号转导的第二信使在细胞调节中起重要作用。许多刺激激活磷脂酶D，其催化磷脂酰胆碱的水解，产生磷脂酸和胆碱。在这里，我们报道了酵母SP 014基因，其对于减数分裂是必需的[Honigberg，S. M.，科尼切拉角& Esposito，R. E.（1992）Genetics 130，703-716]，编码磷脂酶D。SP 014 RNA和蛋白质活性在减数分裂后期前期被诱导，并且该酶具有与哺乳动物磷脂酰肌醇4，5-二磷酸调节的磷脂酶D相似的性质。SP 014的一个不寻常的等位基因的表征定义了对酶催化和调节重要的蛋白质区域。这些结果暗示磷脂酶D信号在调节细胞分化。
Phospholipid metabolism plays an important role in cellular regulation by generating second messengers for signal transduction. Many stimuli activate a phospholipase D, which catalyzes the hydrolysis of phosphatidylcholine, producing phosphatidic acid and choline. Here we report that the yeast SP014 gene, which is essential for meiosis [Honigberg, S. M., Conicella, C. & Esposito, R. E. (1992) Genetics 130, 703-716], encodes a phospholipase D. SP014 RNA and protein activity are induced during late meiotic prophase, and the enzyme has properties similar to mammalian phosphatidylinositol 4,5-bisphosphate-regulated phospholipase D. Characterization of an unusual allele of SP014 defines regions of the protein important for enzyme catalysis and regulation. These results implicate phospholipase D signaling in regulating cellular differentiation.