Decreased expression of miR-9 due to E50K OPTN mutation causes disruption of the expression of BDNF leading to RGC-5 cell apoptosis
Decreased expression of miR-9 due to E50K OPTN mutation causes disruption of the expression of BDNF leading to RGC-5 cell apoptosis
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DOI：
10.3892/mmr.2016.5810
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发表时间：
2016-11-01
影响因子：
3.4
通讯作者：
Yuan, Huiping
中科院分区：
文献类型：
--
作者：
Jiang, Bo;Gao, Lin;Yuan, Huiping
本研究的目的是研究E50 K视神经磷酸酶（OPTN）突变对RGC-5细胞的影响，并确定microRNA-9（miR-9）在该系统中的作用。使用转染的RGC-5细胞来评估E50 K OPTN对miR-9表达的影响，并使用蛋白质印迹分析随后对RGC-5细胞凋亡的破坏。结果显示，E50 K OPTN的表达与E50 K OPTN转染的RGC-5细胞中miR-9水平的显著降低相关。miR-9的E50 K OPTN依赖性减少导致转录抑制因子RE 1沉默转录因子的表达增加，并降低脑源性神经营养因子的表达。因此，E50 K OPTN可能会破坏miR-9的表达，提示E50 K OPTN突变可能导致RGC-5细胞凋亡的潜在机制。
The aims of the present study were to investigate the effect of E50K optineurin (OPTN) mutation on RGC-5 cells and to define the role of microRNA-9 (miR-9) in this system. Transfected RGC-5 cells were used to evaluate the effects of E50K OPTN on the expression of miR-9 and subsequent disruption of RGC-5 cell apoptosis was analyzed using western blotting. The results showed that the expression of E50K OPTN was associated with a marked reduction in the levels of miR-9 in the E50K OPTN-transfected RGC-5 cells. The E50K OPTN-dependent reductions in miR-9 led to increased expression of the transcriptional repressor, RE1-silencing transcription factor and decreased the expression of brain-derived neurotrophic factor. Thus, E50K OPTN may disrupt the expression of miR-9, suggesting a potential mechanism by which E50K OPTN mutation may lead to RGC-5 cell apoptosis.