LUCIFERIN BIOSYNTHESIS IN LARVAL PYREARINUS-TERMITILLUMINANS (COLEOPTERA, ELATERIDAE)

LUCIFERIN BIOSYNTHESIS IN LARVAL PYREARINUS-TERMITILLUMINANS (COLEOPTERA, ELATERIDAE)
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DOI:
10.1016/0305-0491(88)90126-5
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发表时间:
1988-01-01
影响因子:
2.2
通讯作者:
BECHARA, EJH
BECHARA, EJH
中科院分区:
生物学3区
文献类型:
--
作者:
COLEPICOLO, P;PAGNI, D;BECHARA, EJH

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1. 通过在点击甲虫 Pyrearinus termitillusans 幼虫的腹部注射 14C-胱氨酸(前体),然后分离和计数标记的荧光素,研究了荧光素在其幼虫中的生物合成。 2.测量剂量和时间反应;掺入标记的胱氨酸 25 小时后,荧光素的放射化学产率达到 3% 的稳定水平。 3.观察到的时间响应曲线与先前描述的使用苯并噻唑衍生物作为荧光素前体的​​萤火虫的时间响应曲线相似。
1. The biosynthesis of luciferin in larvae of the click beetle Pyrearinus termitilluminans has been studied by injection of 14C-cystine (precursor) in their abdominal segments, followed by isolation and counting labeled luciferin. 2. Dose and time response were measured; the radiochemical yield of luciferin reached a plateau at 3% after 25 hours incorporation of labelled cystine. 3. The observed temporal response curve is similar to that previously described for fireflies using a benzothiazol derivative as luciferin precursor.