Association mapping of cadmium, copper and hydrogen peroxide tolerance of roots and translocation capacities of cadmium and copper in Arabidopsis thaliana.
Association mapping of cadmium, copper and hydrogen peroxide tolerance of roots and translocation capacities of cadmium and copper in Arabidopsis thaliana.
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DOI：
10.1111/j.1399-3054.2009.01286.x
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发表时间：
2009-11
影响因子：
6.4
通讯作者：
T. Tazib;Yuriko Kobayashi;Takashi Ikka;Cheng-Ri Zhao;S. Iuchi;Masatomo Kobayashi;K. Kimura;H. Koyama
中科院分区：
文献类型：
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作者：
T. Tazib;Yuriko Kobayashi;Takashi Ikka;Cheng-Ri Zhao;S. Iuchi;Masatomo Kobayashi;K. Kimura;H. Koyama
以相对根长（胁迫条件下根长相对于对照条件下根长所占比例RRL）和Cd、Cu转运比（茎中金属含量与总金属含量之比）为指标，对90份拟南芥的Cd、Cu和H (2)O(2)耐受性进行关联作图分析。使用分布在整个基因组中的140个snp，通过Q + K方法在单倍体设置下进行关联作图分析，该方法通过将结构化关联(Q)的Q矩阵与亲缘关系(K)相结合来控制群体结构，从而最大限度地减少错误关联的检测。在这些snp与Cd、Cu和H(2)O(2)抗性RRLs之间分别检测到6个、5个和5个显著（-log (10) p值为1.3 >或=）的联系。此外，还发现了6个与Cd和Cu转运能力有关的重要联系。其中，铜、镉耐受性RRL各2个与H(2)O(2)耐受性RRL配位，铜、镉耐受性RRL各1个与其易位比配位。这些因素可能部分解释了拟南芥Cd和Cu耐受性RRLs的表型变异。最后，使用不同的方法来分析个体表型之间的相互作用，即聚类分析，我们发现在携带多个性状的典型加入组中，多个RRLs的抗性snp（单核苷酸多态性）存在预期的分离。关联作图分析检测到的位点几乎没有与先前鉴定的调节性状的关键基因的位点相关联，这表明这可能有助于鉴定决定多品种间变异的遗传因子的复杂结构。
Association mapping analysis of Cd, Cu and H (2)O (2) tolerance, judged by relative root length (RRL: % of root length in stress condition relative to that in control condition), and Cd and Cu translocation ratios (amount of metal in the shoot to the total) were performed using 90 accessions of Arabidopsis thaliana. Using 140 SNPs that were distributed across the genome, association mapping analysis was performed with a haploid setting by the Q + K method, which minimizes detection of false associations by combining the Q-matrix of the structured association (Q) with kinship (K) to control for the population structure. Six, five and five significant (-log (10)P-value is 1.3 > or =) linkages were detected between the SNPs and Cd, Cu and H(2)O(2) resistant RRLs, respectively. In addition, six significant linkages were identified with translocation capacities of Cd and Cu. Among those detected loci, two each of Cu and Cd tolerance RRLs were collocated with those of H(2)O(2) tolerance RRL, while one locus each was detected by Cu and Cd tolerance RRLs that collocated with their translocation ratios. These results suggested that these factors might partly explain the phenotypic variation of tolerance RRLs to Cd and Cu of Arabidopsis thaliana. Finally, using a different approach to analyze interactions between individual phenotypes, namely clustering analysis, we found an expected segregation of resistant SNPs (single-nucleotide polymorphisms) of the multiple RRLs in the typical accession groups carrying multiple traits. Almost none of the loci detected by association mapping analysis were linked to the loci of previously identified critical genes regulating the traits, suggesting that this could be useful to identify complex architecture of genetic factors determining variation among multiple accessions.