Selecting the right gate to identify relevant cells for your assay: a study of thioglycollate-elicited peritoneal exudate cells in mice.
Selecting the right gate to identify relevant cells for your assay: a study of thioglycollate-elicited peritoneal exudate cells in mice.
复制标题
DOI：
10.1186/s13104-017-3019-5
复制
发表时间：
2017-12-06
影响因子：
1.8
通讯作者：
Dos-Santos WLC
中科院分区：
文献类型：
--
作者：
Hermida MDR;Malta R;de S Santos MDPC;Dos-Santos WLC
在这项研究中，我们调查的多样性和调制的白细胞群体中定义的门的大小和粒度在不同的时间点巯基乙酸盐诱导的腹膜炎小鼠。将炎性细胞分布到由细胞大小和粒度定义的数据绘图图的四个区域（R1-R4）中。R1和R2含有尺寸较小的无颗粒细胞，主要包括T（CD 3+）淋巴细胞沿着B（B220+）淋巴细胞。巨噬细胞（F4/80+）是在R3区发现的主要细胞。然而，这些细胞存在于所有区域，尽管R1和R2的频率较低。粒细胞（Gr 1+）主要分布在R3和R4。F4/80+和Gr 1+细胞的广泛分布可能反映了不同巨噬细胞和粒细胞群体的募集和活化状态。基于这些观察，大小和粒度可能有助于巯基乙酸盐引起的腹膜渗出液细胞的分析和分选的初始步骤。然而，发育阶段和细胞活化状态可能会干扰使用大小和粒度作为参数的细胞分离。本文的在线版本（10.1186/s13104-017-3019-5）包含补充材料，可供授权用户使用。
In this study, we investigate the diversity and modulation of leukocyte populations represented in the gates defined by size and granularity at different time points of thioglycollate-induced peritonitis in mouse. The inflammatory cells were distributed into four regions (R1–R4) of a data plot graph defined by cell size and granularity. R1 and R2 contained agranular cells that were small in size and predominately included T (CD3+) lymphocytes along with B (B220+) lymphocytes. Macrophages (F4/80+) were the predominant cells found in the R3 region. However, these cells were present in all regions, albeit at a lower frequency in R1 and R2. Granulocytes (Gr1+) were mainly distributed in R3 and R4. The wide distribution of F4/80+ and Gr1+ cells may reflect the recruitment and activation state of the different macrophage and granulocyte populations. Based on these observations, size and granularity may contribute to an initial step in the analysis and sorting of thioglycollate-elicited peritoneal exudate cells. However, the developmental stage and cell activation state may interfere with cell segregation using size and granularity as parameters. The online version of this article (10.1186/s13104-017-3019-5) contains supplementary material, which is available to authorized users.