Schistosoma mansoni, S-haematobium, and S-japonicum:: early events associated with penetration and migration of schistosomula through human skin
Schistosoma mansoni, S-haematobium, and S-japonicum:: early events associated with penetration and migration of schistosomula through human skin
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DOI：
10.1016/s0014-4894(03)00024-9
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发表时间：
2002-10-01
影响因子：
2.1
通讯作者：
Ramaswamy, K
中科院分区：
文献类型：
--
作者：
He, YX;Chen, L;Ramaswamy, K
曼氏血吸虫童虫的迁飞型。haematobium和S.在皮肤器官培养物中分析了日本血吸虫通过人皮肤的情况。这些研究表明，S. mansoni和S.埃及血蜱在人体皮肤中的迁移模式相似。在感染后的前24小时内，近90%的S. mansoni和S.黑热病只存在于表皮中。大多数童虫仅在48 h后才在真皮中发现，它们似乎在感染后72 h左右到达真皮血管。S.日本血吸虫与其它两种的显著不同之处在于90%以上的寄生虫在感染后的最初24 h内已经到达真皮，而童虫在感染后2 h内存在于真皮血管中。通过宏基因阵列和RT-PCR分析感染后8小时的细胞因子模式的分析显示，在所分析的24种不同细胞因子中，只有IL-1 ra、IL-10和TNF-α在感染S. mansoni和S. haematobium感染后，而S.日本血吸虫感染后，IL-1 β、IL-1 ra、IL-2、IL-6、IL-8、IL-10、IL-15、IL-18和TNF-α显著增加。免疫组化分析显示，表皮片中HLA-DR+细胞聚集在S. mansoni已经进入了人类的皮肤。(C)2003 Elsevier Science（美国）。All rights reserved.
Migratory pattern of schistosomula of Schistosoma mansoni, S. haematobium, and S. japonicum through human skin were analyzed in skin organ cultures. These studies showed that the schistosomula of S. mansoni and S. haematobium has similar migratory patterns through human skin. During the first 24 h after infection nearly 90% of S. mansoni and S. haematobium schistosomula were present only in the epidermis. Majority of the schistosomula were found in the dermis only after 48 h and they appear to reach the dermal vessels around 72 h after infection. Migratory pattern of S. japonicum on the other hand was significantly different from the other two species in that over 90% of the parasites had already reached the dermis within the first 24 h and schistosomula were present in the dermal vessels within 2 h after infection. Analysis of the cytokine pattern at 8 h after infection by a macro gene array and RT-PCR analysis showed that out of 24 different cytokines analyzed only IL-1ra, IL-10, and TNF-alpha were increased in the human skin following infections with S. mansoni and S. haematobium, whereas, after infection with S. japonicum there was significant increases in IL-1beta, IL-1ra, IL-2, IL-6, IL-8, IL-10, IL-15, IL-18, and TNF-alpha. Immunohistochemical analysis of epidermal sheets showed focal accumulation of HLA-DR+ cells in areas where schistosomula of S. mansoni had entered the human skin. (C) 2003 Elsevier Science (USA). All rights reserved.