Fourier transform infrared spectroscopic studies of diabetic rat heart crude membranes
Fourier transform infrared spectroscopic studies of diabetic rat heart crude membranes
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DOI：
10.1155/2003/472834
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发表时间：
2003
期刊：
影响因子：
0.5
通讯作者：
F. Severcan;N. Kaptan;B. Turan
中科院分区：
文献类型：
--
作者：
F. Severcan;N. Kaptan;B. Turan
中红外光谱在糖尿病组织中的应用将突出这项技术在医学研究中的前景。所呈现的实例主要将集中于糖尿病大鼠心脏粗膜。链脲佐菌素（STZ）诱导大鼠糖尿病（DM）是研究I型糖尿病最常用的实验模型之一。通过对红外光谱中峰位、带宽和谱带强度的变化进行分析，得到了有价值的结构和功能信息，这些信息可能具有诊断价值。在糖尿病膜中，观察到脂质顺序和脂质浓度显着增加。当饱和脂质的浓度增加时，不饱和脂质的浓度降低。此外，酰胺I谱带的加宽和1640 cm-1附近新谱带的出现以及酰胺I与酰胺II比率的降低表明糖尿病膜蛋白质中发生了结构变化。还观察到低聚糖谱带（1042 cm−1）的显著增加和烯烃伸缩谱带（3012 cm−1）的减少。
Application of mid infrared spectroscopy in diabetic tissues will be presented which highlight the promise of this technique in medical research. Examples presented mainly will focus on diabetic rat heart crude membranes. Diabetes mellitus (DM) was induced in rats by streptozotocin (STZ) injection that is one of the most popular experimental models for the study of type I diabetes. The shift in the peak positions, bandwidths and the intensity of the bands in FTIR spectra were analyzed to have valuable structural and functional information, which may have diagnostic value. In the diabetic membranes significant increase was observed in lipid order and lipids concentration. While the concentration of saturated lipids increases, the concentration of unsaturated lipids decreases. In addition, broadening of the amide I bands and the appearance of the new band around 1640 cm−1 together with the decrease in the amide I to amide II ratio indicate the structural changes occurring in proteins of diabetic membranes. A dramatic increase in oligosaccarite band (1042 cm−1) and a decrease in olefinic stretching band (3012 cm−1), were also observed.